Plant promoter sequences and methods of use thereof

{
  "publication_number": "US2002046415A1",
  "country": "US",
  "kind": "A1",
  "title": "Plant promoter sequences and methods of use thereof",
  "abstract": "The invention relates to nucleic acid sequences isolated from sugarcane and to methods of using them. In particular, the inventions relates to nucleotide sequences which are derived from sugarcane polyubiquitin genes and which are capable of directing constitutive expression of a nucleic acid sequence of interest that is operably linked to the sugarcane polyubiquitin nucleotide sequences. The sugarcane polyubiquitin nucleotide sequences are useful in regulating expression of a nucleic acid sequence of interest in monocotyledonous and dicotyledonous plants.",
  "claims": [
    "1. A substantially purified nucleic acid sequence comprising a nucleotide sequence selected from the group consisting of SEQ ID NO:7, the complement of SEQ ID NO:7, homologs of SEQ ID NO:7, homologs of the complement of SEQ ID NO:7; SEQ ID NO:10, the complement of SEQ ID NO:10, homologs of SEQ ID NO:10, and homologs of the complement of SEQ ID NO:10. 2. The substantially purified nucleic acid sequence of claim 1, wherein said nucleotide sequence is characterized by having promoter activity. 3. The substantially purified nucleic acid sequence of claim 2, wherein said promoter activity is constitutive. 4. A substantially purified nucleic acid sequence comprising a portion of a nucleotide sequence selected from the group consisting of SEQ ID NO:7 and the complement thereof. 5. The substantially purified nucleic acid sequence of claim 4, wherein said portion is characterized by having promoter activity. 6. The substantially purified nucleic acid sequence of claim 5, wherein said promoter activity is constitutive. 7. The substantially purified nucleic acid sequence of claim 4, wherein said portion comprises the nucleotide sequence selected from the group consisting of the nucleotides from 1 to 242, from 245 to 787, from 788 to 1020, from 1021 to 1084, from 1085 to 1168, from 1169 to 1173, from 1174 to 1648, from 1649 to 1802, from 1 to 377, from 378 to 442, and from 443 to 1802. 8. A substantially purified nucleic acid sequence comprising a portion of a nucleotide sequence selected from the group consisting of SEQ ID NO:10 and the complement thereof. 9. The substantially purified nucleic acid sequence of claim 8, wherein said portion is characterized by having promoter activity. 10. The substantially purified nucleic acid sequence of claim 9, wherein said promoter activity is constitutive. 11. The substantially purified nucleic acid sequence of claim 8, wherein said portion comprises the nucleotide sequence selected from the group consisting of the nucleotides 1 to 3600, from 3602 to 3612, from 3614 to 3688, from I to 2248, from 2249 to 2313, from 2314 to 3688, and from 1671 to 2248. 12. A substantially purified nucleic acid sequence comprising the EcoRI/XbaI fragment isolated from plasmid pubi4-GUS contained in Escherichia coli cells deposited as NRRLB-30115, the complement of said fragment, homologs of said fragment, and homologs of the complement of said fragment. 13. A substantially purified nucleic acid sequence comprising the HindIII/XbaI fragment isolated from plasmid pubi9-GUS contained in Escherichia coli cells deposited as NRRLB-30116, the complement of said fragment, homologs of said fragment, and homologs of the complement of said fragment. 14. A substantially purified nucleic acid sequence comprising a portion of the EcoRI/XbaI fragment isolated from plasmid pubi4-GUS contained in Escherichia coli cells deposited as NRRLB-30115, and the complement of said fragment. 15. A substantially purified nucleic acid sequence comprising a portion of the HindIII/XbaI fragment isolated from plasmid pubi9-GUS contained in Escherichia coli cells deposited as NRRLB-30116, and the complement of said fragment. 16. A transgenic plant cell comprising a nucleic acid sequence comprising a nucleotide sequence selected from the group consisting of SEQ ID NO:7, the complement of SEQ ID NO:7, homologs of SEQ ID NO:7, homologs of the complement of SEQ ID NO:7; SEQ ID NO:10, the complement of SEQ ID NO:10, homologs of SEQ ID NO:10, and homologs of the complement of SEQ ID NO:10, wherein said nucleotide sequence is operably linked to a nucleic acid sequence of interest. 17. A transgenic plant cell comprising a nucleic acid sequence comprising a portion of a nucleotide sequence selected from the group consisting of SEQ ID NO:7, the complement of SEQ ID NO:7, SEQ ID NO:10, and the complement of SEQ ID NO:10. 18. A method for expressing a nucleic acid sequence of interest in a plant cell, comprising: a) providing: i) a plant cell; ii) a nucleic acid sequence of interest; and iii) a nucleotide sequence selected from the group consisting of SEQ ID NO:7, the complement of SEQ ID NO:7, homologs of SEQ ID NO:7, homologs of the complement of SEQ ID NO:7; SEQ ID NO:10, the complement of SEQ ID NO:10, homologs of SEQ ID NO:10, and homologs of the complement of SEQ ID NO:10; b) operably linking said nucleic acid sequence of interest to said nucleotide sequence to produce a transgene; and c) introducing said transgene into said plant cell to produce a transgenic plant cell under conditions such that said nucleic acid sequence of interest is expressed in said transgenic plant cell. 19. The method of claim 18, further comprising d) identifying said transgenic plant cell. 20. The method of claim 18, further comprising d) regenerating transgenic plant tissue from said transgenic plant cell. 21. The method of claim 18, further comprising d) regenerating a transgenic plant from said transgenic plant cell. 22. A method for expressing a nucleic acid sequence of interest in a plant cell, comprising: a) providing: i) a plant cell; ii) a nucleic acid sequence of interest; and iii) a portion of a nucleotide sequence selected from the group consisting of SEQ ID NO:7 and the complement thereof; b) operably linking said nucleic acid sequence of interest to said portion of a nucleotide sequence to produce a transgene; and c) introducing said transgene into said plant cell to produce a transgenic plant cell under conditions such that said nucleic acid sequence of interest is expressed in said transgenic plant cell. 23. The method of claim 22, wherein said portion comprises the nucleotide sequence selected from the group consisting of the nucleotides from 1 to 242, from 245 to 787, from 788 to 1020, from 1021 to 1084, from 1085 to 1168, from 1169 to 1173, from 1174 to 1648, from 1649 to 1802, from 1 to 377, from 378 to 442, and from 443 to 1802. 24. A method for expressing a nucleic acid sequence of interest in a plant cell, comprising: a) providing: i) a plant cell; ii) a nucleic acid sequence of interest; and iii) a portion of a nucleotide sequence selected from the group consisting of SEQ ID NO:10 and the complement thereof; b) operably linking said nucleic acid sequence of interest to said portion of a nucleotide sequence to produce a transgene; and c) introducing said transgene into said plant cell to produce a transgenic plant cell under conditions such that said nucleic acid sequence of interest is expressed in said transgenic plant cell. 25. The method of claim 24, wherein said portion comprises the nucleotide sequence selected from the group consisting of the nucleotides 1 to 3600, from 3602 to 3612, from 3614 to 3688, from 1 to 2248, from 2249 to 2313, from 2314 to 3688, and from 1671 to 2248."
  ],
  "cpc": [
    "C07K 14/415",
    "C07K 14/00",
    "C12N 15/8216"
  ],
  "assignees": [
    "US AGRICULTURE"
  ],
  "filing_date": "2001-05-24",
  "publication_date": "2002-04-18",
  "priority_date": "1998-03-19",
  "application_number": "US-86615301-A",
  "family_id": "26760912"
}