S-adenosyl-L-homocystein hydrolase promoter

{
  "publication_number": "US6037524A",
  "country": "US",
  "kind": "A",
  "title": "S-adenosyl-L-homocystein hydrolase promoter",
  "abstract": "PCT No. PCT/GB96/00882 Sec. 371 Date Oct. 9, 1997 Sec. 102(e) Date Oct. 9, 1997 PCT Filed Apr. 10, 1996 PCT Pub. No. WO96/32488 PCT Pub. Date Oct. 17, 1996A promoter derived from an SHH gene, especially the SHH gene of Arabidopsis thaliana which is capable of directing expression on a variety of operator genes in both monocotyledonous and dicotyledonous plants. The promoter of the invention may be used for directing expression of pathogen resistance genes to disease or wound sites.",
  "claims": [
    "1. An isolated promoter derived from the S-adenosyl-L-homocysteine hydrolase gene of A. thaliana.",
    "2. An isolated promoter having the nucleotide sequence of that part of SEQ ID NO 3 which is upstream of the methionine codon as shown in FIGS. 5A-5C.",
    "3. A DNA construct comprising a promoter as claimed in claim 1 or claim 2 operably linked to a gene.",
    "4. A DNA construct as claimed in claim 3 wherein said gene is selected from the group consisting of a gene encoding S-adenosyl-L-homocysteine hydrolase, an antifungal protein gene, a selectable marker gene, NptII, a kanamycin resistance gene, a phosphinothricin resistance gene, the phosphinothricin acetyl transferase (PAT) gene, the glucuronidase (GUS) reporter gene, and the luciferase (LUC) reporter gene.",
    "5. A vector comprising a DNA construct as claimed in claim 3 or claim 4.",
    "6. A vector as claimed in claim 5 which is a binary agrobacterium vector or a direct DNA delivery vector.",
    "7. A plant cell transformed with a vector as claimed in claim 5.",
    "8. A genetically transformed plant or part thereof having stably incorporated into the genome the DNA construct as claimed in claim 3 or claim 4.",
    "9. A plant cell or genetically transformed plant according to claim 7 or claim 8, wherein the plant is wheat, maize, oil seed rape, potato, tomato, banana or tobacco.",
    "10. A method of increasing the resistance of a plant to infection by a pathogenic organism, the method comprising transforming the plant with a vector comprising a promoter according to claim 1 or claim 2 operably linked to a gene conferring resistance to the pathogenic organism.",
    "11. A method as claimed in claim 10 wherein the pathogenic organism is a fungus and the gene encodes an antifungal protein.",
    "12. A genetically transformed plant or part thereof according to claim 8 which is a cell, protoplast or seed."
  ],
  "description_excerpt": "This application is a 371 of PCT/GB/00882 filed Apr. 10, 1996.\n\nThe present invention relates to a promoter sequence capable of giving a high level of expression within plant cells. In particular, it relates to a promoter derived from a gene encoding S-adenosyl-L-homocysteine hydrolase (SHH).\n\nPromoters control the spatial and temporal expression of genes by modulating their level of transcription. Early approaches to genetically engineered crop plants utilised strong constitutive promoters to drive the expression of foreign genes. As strategies in plant biotechnology have become more sophisticated, specific promoters have been used to target transgene expression to a particular tissue or to a particular developmental stage. The promoter of the present invention is especially versatile as it can be used either to give constitutive expression of a gene or to target increased levels of gene expression at sites of wounding or pathogen invasion.\n\nSHH was first described, in rat liver extracts, as the activity responsible for the reversible hydrolysis of S-adenosyl-L-homocysteine (SAH) to adenosine and homocysteine by the cleavage of a thioether bond in SAH [de la Haba, G. and Cantoni, G. L. (1959). J. Biol. Chem. 234, 603-608].\n\nSAH is formed as a direct product of transmethylation reactions involving S-adenosyl-L-methionine (SAM) [Cantoni, G. L. and Scarano, E. (1954). J. Am. Chem. Soc. 76, 4744] and is known to be a potent inhibitor of most SAM mediated methyltransfer reactions. Therefore SAH is converted to homocysteine and adenosine by SHH as shown schematically below:",
  "cpc": [
    "C12N 15/8216",
    "C12N 15/8243",
    "C12N 15/8282",
    "C12N 9/14"
  ],
  "ipc": [
    "A01H 5/00",
    "A01H 6/20",
    "A01H 6/46",
    "A01H 6/82",
    "C12N 15/00",
    "C12N 15/09",
    "C12N 15/29",
    "C12N 15/55",
    "C12N 15/82",
    "C12N 15/84",
    "C12N 5/10",
    "C12N 9/14"
  ],
  "assignees": [
    "Zeneca Ltd"
  ],
  "inventors": [
    "Andrew James Greenland",
    "John Draper",
    "Mark Skipsey",
    "Simon Warner"
  ],
  "filing_date": "1996-04-10",
  "publication_date": "2000-03-14",
  "grant_date": "2000-03-14",
  "priority_date": "1995-04-10",
  "application_number": "US-93089497-A",
  "family_id": "10772814",
  "cited_by_count": 7,
  "citations": [
    "EP0039225A1",
    "EP0492536A2",
    "JPH04258292A",
    "WO1993019188A1"
  ]
}