Composition for preventing or treating cancer comprising extracts of anemone raddeana, lonicera species, and aralia elata containing high concentration of antitumor saponins, and method for preparing same

{
  "publication_number": "US2021236576A1",
  "country": "US",
  "kind": "A1",
  "title": "Composition for preventing or treating cancer comprising extracts of anemone raddeana, lonicera species, and aralia elata containing high concentration of antitumor saponins, and method for preparing same",
  "abstract": "The present invention relates to a composition for preventing or treating cancer, comprising extracts of Anemone raddeana and Lonicera species containing a high concentration of antitumor saponins, and a method for preparing the same, wherein saponins contained in the extracts of Anemone raddeana and Lonicera species, are converted to anticancer saponins having high antitumor activity, thereby producing extracts containing a high concentration of the antitumor saponins, and the extracts thus prepared have been confirmed to show desirable antitumor activity. Accordingly, the extracts containing a high concentration of antitumor saponins of the present invention are promising for use in the development of drugs for cancer treatment.",
  "claims": [
    "1. A pharmaceutical composition comprising a plant extract as an active ingredient for prevention or treatment of cancer, the plant extract being prepared by a process comprising: i) a first step of adding distilled water to at least one plant selected from the group consisting of Anemone raddeana and Pulsatilla koreana and grinding to prepare a plant medium; ii) a second step of adding an organic solvent to at least one plant selected from the group consisting of Lonicera species, Anemone raddeana, Pulsatilla koreana and Aralia elata to prepare an extract thereof; iii) a third step of combining the medium of the plants in the first step with the extract in the second step and fermenting the combination at 37° C. while stirring; iv) a fourth step of adding an organic solvent to the fermented product obtained by fermentation in the third step to form fractions; v) a fifth step of subjecting the fractions of the fourth step to column chromatography to obtain eluates; and vi) a sixth step of adding an organic solvent to the eluates of the fifth step to obtain fractions and drying the fractions to obtain the plant extract. 2. The pharmaceutical composition of claim 1, wherein the plant medium in the first step is prepared by primarily grinding the at least one plant in distilled water in a mixer; and secondarily performing grinding the by sonication. 3. The pharmaceutical composition of claim 1, wherein the plant in the first step is Anemone raddeana, and the plant in the second step is Lonicera species, or a combination of Lonicera species and Aralia elata. 4. The pharmaceutical composition of claim 1, wherein the Anemone raddeana and Lonicera species an anticancer saponin content of the plant extract is 15 to 110 fold improved in anticancer saponin content. 5. The pharmaceutical composition of claim 4, wherein the anticancer saponin is at least one selected from the group consisting of hederagenin 3-O-β-D-glucopyranosyl-(1→4)-β-D-glucopyranosyl-(1→3)-α-L-rhamnopyranosyl-(1→2)-α-L-arabinopyranoside, oleanolic acid 3-O-α-L-rhamnopyranosyl-(1→2)-[β-D-glucopyranosyl-(1→4)]-α-L-arabinopyranoside, and hederagenin 3-O-β-D-glucopyranosyl-(1→3)-α-L-rhamnopyranosyl-(1→2)-α-L-arabinopyranoside. 6. The pharmaceutical composition of claim 1, further comprising an anticancer agent. 7. The pharmaceutical composition of claim 1, wherein the cancer is a solid cancer. 8. The pharmaceutical composition of claim 1, wherein the plant in the first step is Pulsatilla koreana, and the plant in the second step is Lonicera species and Anemone raddeana. 9. A process for preparing a plant extract comprising: i) a first step of adding distilled water to at least one plant selected from the group consisting of Anemone raddeana and Pulsatilla koreana and grinding to prepare a plant medium; ii) a second step of adding an organic solvent to at least one plant selected from the group consisting of Lonicera species, Anemone raddeana, Pulsatilla koreana and Aralia elata to prepare an extract thereof; iii) a third step of combining the medium of the plants in the first step with the extract in the second step and fermenting the combination at 37° C. while stirring; iv) a fourth step of adding an organic solvent to the fermented product obtained by fermentation in the third step to form fractions; v) a fifth step of subjecting the fractions of the fourth step to column chromatography to obtain eluates; and vi) a sixth step of adding an organic solvent to the eluates of the fifth step to obtain fractions and drying the fractions to obtain the plant extract."
  ],
  "description_excerpt": "The present disclosure relates to a composition comprising an Anemone raddeana and Lonicera species extract for prevention or treatment of cancer and, more particularly, to a composition for prevention or treatment of cancer, the composition comprising an Anemone raddeana and Lonicera species extract containing a high concentration of anticancer saponins, alone or in combination with an Aralia elata extract or a Pulsatilla sp. extract, and a preparation method therefor.\n\nCancers, academically called neoplasia, are characterized by uncontrolled cell growth. A group of cells that have undergone abnormal growth forms a mass, called a tumor, which invades to neighboring tissues and, in severe cases, may be metastasized to other organs in the body. Cancers affect all tissues and organs across the body at various prevalence rates.\n\nThe types of cancer may be classified according to tissues and organs in which cancer cells first develop, and according to morphologies and origins of cancer cells. Lung cancer, gastric cancer, colorectal cancer, cervical cancer, and the like are often referred to on the basis of the organs in which the tumor is first developed. In addition, cancers may be largely divided into connective tissue tumors, epithelial tumors, adenocarcinoma, and the like in an aspect of the origin of cancer cells. In South Korea, the most common cancer is thyroid cancer, followed by gastric cancer, colorectal cancer, and lung cancer, in the order of incidence.",
  "cpc": [
    "A61K 36/71",
    "A61K 2236/15",
    "A61K 2236/19",
    "A61K 2236/331",
    "A61K 2236/39",
    "A61K 2236/55",
    "A61K 31/365",
    "A61K 31/704",
    "A61K 36/25",
    "A61K 36/355",
    "A61K 45/06",
    "A61P 35/00"
  ],
  "ipc": [
    "A61K 31/704",
    "A61K 36/25",
    "A61K 36/355",
    "A61K 36/71",
    "A61P 35/00"
  ],
  "inventors": [
    "Byung Zun Ahn",
    "Jae Seung ROH"
  ],
  "filing_date": "2019-03-12",
  "publication_date": "2021-08-05",
  "priority_date": "2018-05-04",
  "application_number": "US-201917053040-A",
  "family_id": "68386577",
  "cited_by_count": 2,
  "citations": [
    "KR20060092158A"
  ]
}