Cell cryopreservation tool

{
  "publication_number": "US9516876B2",
  "country": "US",
  "kind": "B2",
  "title": "Cell cryopreservation tool",
  "abstract": "A cell cryopreservation tool has a cell holding member having a body part and a cell holding part and a tubular accommodation member, closed at one end thereof, which is capable of accommodating the cell holding member. The cell holding part of the cell holding member has a long and narrow cell attaching and holding portion. The cell attaching and holding portion has a heat conductor extended in a longitudinal direction thereof. When the cell holding member is accommodated in the tubular accommodation member, the heat conductor is capable of contacting an inner surface of the tubular accommodation member.",
  "claims": [
    "1. A cell cryopreservation tool comprising: a cell holding member having a body part formed of a liquid nitrogen-resistant material and a cell holding part formed of said liquid nitrogen-resistant material; an accommodation tube, closed at one end thereof, which is capable of accommodating said cell holding member, and wherein said accommodation tube has a tubular body formed of said liquid nitrogen-resistant material; said cell holding part of said cell holding member has an elongated cell attaching and holding portion; said cell attaching and holding portion has a first metal heat conductor extending in a longitudinal direction thereof and provided at one side or both sides of said cell attaching and holding portion; said first metal heat conductor has an exposed portion exposed from said cell holding member; said accommodation tube has a second metal heat conductor and a distal-end closed portion to prevent cooling medium from entering; said second metal heat conductor has an inner side exposed part exposed to an inner space of said accommodation tube; and when said cell holding member is accommodated in said accommodation tube, said exposed portion of said first metal heat conductor of said cell holding member contacts said inner exposed part of said second metal heat conductor in said accommodation tube.",
    "2. The cell cryopreservation tool according to claim 1, wherein said second metal heat conductor has an outer side exposed part exposed to an outer space of said accommodation tube.",
    "3. The cell cryopreservation tool according to claim 1, wherein said first metal heat conductor of said cell holding member extends from a distal portion of said cell attaching and holding portion to a proximal side of said cell attaching and holding portion.",
    "4. The cell cryopreservation tool according to claim 1, wherein said second metal heat conductor is provided at a distal portion of said accommodation tube.",
    "5. The cell cryopreservation tool according to claim 1, wherein said second metal heat conductor is provided on a side surface of said accommodation tube.",
    "6. The cell cryopreservation tool according to claim 1, wherein said first metal heat conductor is provided at both sides of said cell attaching and holding portion in such a way that said first metal heat conductor is extended from a distal portion of said cell attaching and holding portion to a proximal side of said cell attaching and holding portion.",
    "7. The cell cryopreservation tool according to claim 1, wherein said cell attaching and holding portion has a cell accommodation concave portion; and said first metal heat conductor is disposed essentially at one side or both sides of a portion of said cell attaching and holding portion where said cell accommodation concave portion is formed.",
    "8. The cell cryopreservation tool according to claim 1, wherein said cell holding part has a rectangular cross section.",
    "9. The cell cryopreservation tool according to claim 1, wherein said liquid nitrogen-resistant material is a synthetic resin, and wherein said synthetic resin is 3-polyethylene fluoride, low-density polyethylene, medium-density polyethylene, high-density polyethylene, polycarbonate, nylon, polysulfone, polyester, polystyrene, polyimide, ultra-high-molecular weight polyethylene, ethylene-vinyl acetate copolymer, or laminates of films formed of said synthetic resin.",
    "10. The cell cryopreservation tool according to claim 1, wherein said cell holding part is transparent or semitransparent.",
    "11. The cell cryopreservation tool according to claim 1, wherein said cell holding part has a width of 0.4 mm to 1.0 mm and a length of 5 mm to 30 mm, and wherein said body part has a length of 20 mm to 100 mm.",
    "12. The cell cryopreservation tool according to claim 1, wherein said tubular body is transparent or semitransparent."
  ],
  "description_excerpt": "The present invention relates to a cell cryopreservation tool used in cryopreservation cells such as mammalian ova, eggs such as embryos, sperms, and stem cells such as hematopoietic stem cells, pluripotent stem cells, and the like.\n\nCryopreservation the mammalian embryo enables conservation of hereditary resources of specific systems and kinds. It is effective for maintaining animals standing on the brink of ruin. It is useful for infertility treatment.\n\nAs a method for cryopreservation mammalian embryos, as disclosed in patent document 1 (Japanese Patent Application Laid-Open Publication No. 2000-189155), there is proposed a method for cryopreservation mammalian embryos that mammalian embryos or ova are bonded to the inner surface of the cryopreservation container such as the sterilized frozen straw, frozen vial or frozen tube by using a vitrifying liquid in an amount minimum and enough to enclose the mammalian embryos or the ova therewith. The cryopreservation container is sealed and rapidly cooled by bringing the cryopreservation container into contact with liquid nitrogen. In the thawing method, the cryopreservation container stored in the above method is taken out of the liquid nitrogen and one end thereof is opened. A diluted liquid of 33 to 39 degrees C. is injected directly into the container to thaw the mammalian embryos or the ova and dilute the vitrifying liquid. This method eliminates a possibility that the mammalian embryos or the ova are infected with a disease through viruses or bacteria and is capable of storing them at a high survival rate and thawing them and diluting the vitrifying liquid.",
  "cpc": [
    "A01N 1/145",
    "A01N 1/0257",
    "A01N 1/0268",
    "A01N 1/142",
    "A01N 1/147",
    "G01N 1/42"
  ],
  "ipc": [
    "A01N 1/02",
    "G01N 1/42"
  ],
  "assignees": [
    "Kitazato Biopharma Co Ltd"
  ],
  "inventors": [
    "Futoshi Inoue"
  ],
  "filing_date": "2012-10-01",
  "publication_date": "2016-12-13",
  "grant_date": "2016-12-13",
  "priority_date": "2011-10-04",
  "application_number": "US-201214349244-A",
  "family_id": "48043679",
  "cited_by_count": 16,
  "citations": [
    "JPH01277485A",
    "JP2000189155A",
    "JP2002315573A",
    "WO2002085110A1",
    "CN1774501A",
    "US20040259072A1",
    "JP2004329202A",
    "CN101087658A",
    "US20080038155A1",
    "WO2007036628A1",
    "US20080233633A1",
    "US20090123992A1",
    "WO2011070973A1",
    "US20110196358A1"
  ]
}