Compositions and methods for 3-hydroxypropionic acid production

{
  "publication_number": "US10633664B2",
  "country": "US",
  "kind": "B2",
  "title": "Compositions and methods for 3-hydroxypropionic acid production",
  "abstract": "The present application discloses genetically modified yeast cells comprising an active 3-HP fermentation pathway, and the use of these cells to produce 3-HP.",
  "claims": [
    "1. A genetically modified yeast cell selected from the group consisting of I. orientalis, C. lambica, and S. bulderi, the genetically modified yeast cell comprising an exogenous BAAT or gabT gene encoding an enzymatically active polypeptide with at least 85% sequence identity to an amino acid sequence selected from SEQ ID NOs: 20, 21, and 24.",
    "2. The genetically modified yeast cell of claim 1, wherein the BAAT or gabT gene encodes a polypeptide having at least 90% sequence identity to the amino acid sequence selected from SEQ ID Nos: 20, 21, and 24.",
    "3. The genetically modified yeast cell of claim 1, wherein the BAAT or gabT gene encodes a polypeptide having at least 95% sequence identity to the amino acid sequence selected from SEQ ID Nos: 20, 21 and 24.",
    "4. The genetically modified yeast cell of claim 1, wherein the BAAT or gabT gene encodes a polypeptide having at least 97% sequence identity to the amino acid sequence selected from SEQ ID Nos: 20, 21, and 24.",
    "5. The genetically modified yeast cell of claim 1, wherein the BAAT or gabT gene encodes a polypeptide having at least 98% sequence identity to the amino acid sequence selected from SEQ ID Nos: 20, 21, and 24."
  ],
  "description_excerpt": "3-hydroxypropionic acid (3-HP) is a three carbon carboxylic acid identified by the U.S. Department of Energy as one of the top 12 high-potential building block chemicals that can be made by fermentation. Alternative names for 3-HP, which is an isomer of lactic (2-hydroxypropionic) acid, include ethylene lactic acid and 3-hydroxypropionate. 3-HP is an attractive renewable platform chemical, with 100% theoretical yield from glucose, multiple functional groups that allow it to participate in a variety of chemical reactions, and low toxicity. 3-HP can be used as a substrate to form several commodity chemicals, such as 1,3-propanediol, malonic acid, acrylamide, and acrylic acid. Acrylic acid is a large-volume chemical (>7 billion lbs/year) used to make acrylate esters and superabsorbent polymers, and is currently derived from catalytic oxidation of propylene. Fermentative production of 3-HP would provide a sustainable alternative to petrochemicals as the feedstock for these commercially-significant chemicals, thus reducing energy consumption, US dependence on foreign oil, and the production of greenhouse gases.\n\nBacteria can be used to ferment sugars to organic acids. However, bacteria present certain drawbacks for large-scale organic acid production. As organic acids are produced, the fermentation medium becomes increasingly acidic. Lower pH conditions are actually preferable, because the resultant product is partially or wholly in the acid form.",
  "cpc": [
    "C12N 15/52",
    "C12N 1/18",
    "C12N 15/81",
    "C12N 15/815",
    "C12N 9/0006",
    "C12N 9/1096",
    "C12N 9/88",
    "C12N 9/93",
    "C12P 7/42",
    "C12P 7/52",
    "C12Y 101/01059",
    "C12Y 206/01001",
    "C12Y 206/01018",
    "C12Y 206/01019",
    "C12Y 401/01031"
  ],
  "ipc": [
    "C12N 1/18",
    "C12N 15/52",
    "C12N 15/81",
    "C12N 9/00",
    "C12N 9/04",
    "C12N 9/10",
    "C12N 9/88",
    "C12P 7/42",
    "C12N 1/20"
  ],
  "assignees": [
    "Cargill Inc"
  ],
  "inventors": [
    "Holly Jessen",
    "Brian Rush",
    "Jeanette Huryta",
    "Beth Mastel",
    "Alan Berry",
    "Debbie Yaver",
    "Michael Catlett",
    "Michelle Barnhart"
  ],
  "filing_date": "2019-04-15",
  "publication_date": "2020-04-28",
  "grant_date": "2020-04-28",
  "priority_date": "2010-11-22",
  "application_number": "US-201916384150-A",
  "family_id": "45099210",
  "cited_by_count": 4,
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