Apparatus for processing samples containing biological cells

{
  "publication_number": "US10907129B2",
  "country": "US",
  "kind": "B2",
  "title": "Apparatus for processing samples containing biological cells",
  "abstract": "An apparatus and a method for the automated processing of samples containing biological cells - in particular, of blood samples or other cell samples is provided. The apparatus has a sample receiving device configured to receive samples, an auxiliary material receiving device configured to receive auxiliary materials, a sample carrier receiving device configured to receive sample carriers, a discharge device configured to discharge samples from a discharge position, and a capture device configured to capture discharged samples in a capture position to obtain prepared samples, and at least one temperature control device for controlling the temperature of at least one of the sample carriers. The distance between the discharge position and the capture position can be adjusted to a prespecified height. The method includes wetting a temperature-controlled sample carrier with at least one auxiliary material and discharging the sample onto the wetted temperature-controlled sample carrier from a prespecified height.",
  "claims": [
    "1. An apparatus for the automated processing of a sample containing biological cells, the apparatus comprising: a temperature-controlled storage area; a working area; a sample receiving device configured to receive the sample; an auxiliary material receiving device configured to receive auxiliary materials; a sample carrier receiving device configured to receive sample carriers; a discharge device configured to discharge the sample from a discharge position; at least one temperature control device configured to control a temperature of at least one of the sample carriers, and a capture device configured to capture the sample discharged from the discharge position in a capture position to obtain a prepared sample, and a distance between the discharge position and the capture position being adjusted to a prespecified height of between 0.1 to 800 mm, the capture device having a holder configured to receive at least one of the sample carriers, said holder being configured such that the at least one sample carrier is arranged in the capture position to obtain the discharged sample.",
    "2. The apparatus according to claim 1, wherein the sample containing the biological cells is a blood sample or another cell sample.",
    "3. The apparatus according to claim 1, further comprising a housing in which components of the apparatus are arranged, wherein in the housing, physical and/or chemical conditions are set which differ from the conditions of the environment.",
    "4. The apparatus according to claim 1, wherein the discharge device includes a pipetting unit with a pipetting head movable at least along one axis.",
    "5. The apparatus according to claim 1, wherein the temperature-controlled storage area includes at least one of the auxiliary material receiving device and the sample carrier receiving device arranged therein.",
    "6. The apparatus according to claim 1, wherein the working area includes at least the discharge device and the capture device arranged therein.",
    "7. The apparatus according to claim 1, wherein: the temperature controlled sample receiving device is arranged in the working area or in the storage area.",
    "8. The apparatus according to claim 1, further comprising a wetting device configured to wet the sample carriers with at least one of the auxiliary materials.",
    "9. The apparatus according to claim 1, further comprising an analysis unit configured to analyze the prepared sample.",
    "10. The apparatus according to claim 1, wherein: the capture device has an actuator configured to adjust a prespecified angle of inclination of the at least one sample carrier, and the angle of inclination of the at least one sample carrier is adjustable to a value between 0 and 90° relative to a horizontal plane."
  ],
  "description_excerpt": "The disclosure relates to an apparatus and a method for processing samples containing biological cells - in particular, blood samples or cell samples.\n\nThe importance of genetic and/or molecular biological analyses is growing in the life sciences and the medical field. Particularly in the areas of prognosis, diagnosis, and documentation of, for example, genetic defects or tumor diseases, methods for preparing cell samples, with the aim of making cell components analytically accessible, have become indispensable.\n\nPart of this analysis is based on the destruction of the cell membrane by dropping a preprocessed cell sample on a sample carrier. This breaks the cell membrane, and emerging cell components - including chromosomes - are available for subsequent procedures such as staining, and for analysis steps such as an image analysis, for example. The manual preparation of a cell sample for analysis of components of the cell sample usually includes the following steps:\n\n(i) Sample preparation: The cell sample with cells containing cell components to be subjected to analysis is usually available in the form of raw sample material. First, it is necessary to obtain the cell sample from the raw sample material. Depending on the cell type, this step can require different procedures. However, it generally consists of enriching and purifying the raw sample material by, for example, centrifuging and optionally further steps such as culturing the cells to generate the cell sample which will be analyzed.",
  "cpc": [
    "C12M 45/02",
    "C12M 29/06",
    "C12M 33/04",
    "C12M 37/02",
    "C12M 41/14",
    "C12M 41/18",
    "C12M 45/06",
    "C12M 45/20"
  ],
  "ipc": [
    "C12M 1/00",
    "C12M 1/02",
    "C12M 1/12",
    "C12M 1/26",
    "C12M 1/33"
  ],
  "assignees": [
    "ALS Automated Lab Solutions GmbH"
  ],
  "inventors": [
    "Matthias Opel",
    "David Unglaub",
    "Jens Eberhardt",
    "Gerd Bornmann"
  ],
  "filing_date": "2019-04-28",
  "publication_date": "2021-02-02",
  "grant_date": "2021-02-02",
  "priority_date": "2016-10-28",
  "application_number": "US-201916396762-A",
  "family_id": "60381987",
  "cited_by_count": 2,
  "citations": [
    "EP0633207A1",
    "US5891734A",
    "US6122396A",
    "WO2000013609A2",
    "US20070053513A1",
    "US7974714B2",
    "US6850252B1",
    "US7813822B1",
    "WO2003008934A1",
    "US20030103662A1",
    "EP1502649A1",
    "US9579255B2",
    "US7783383B2",
    "US20150250678A1",
    "US9043019B2",
    "US20110208350A1",
    "US7930066B2",
    "US20100198392A1",
    "US8457781B2",
    "US20140148944A1",
    "US9020634B2",
    "US20110046775A1",
    "US8943785B2",
    "US20110047042A1",
    "US20130252796A1",
    "US9457359B2",
    "CN202297580U",
    "US20190249133A1",
    "US20190259108A1"
  ]
}