Living cell cryopreservation tool

{
  "publication_number": "US9538746B2",
  "country": "US",
  "kind": "B2",
  "title": "Living cell cryopreservation tool",
  "abstract": "A living cell cryopreservation tool has a body part formed of a cold-resistant material and a living cell holding part formed of the cold-resistant material. The living cell holding part has a long and narrow living cell attaching and holding portion. The living cell attaching and holding portion has a plurality of living cell accommodation concave portions formed in a longitudinal direction thereof and a plurality of excess cryopreservation liquid discharge passages communicating with the living cell accommodation concave portions.",
  "claims": [
    "1. A living cell cryopreservation tool comprising: a body part and a living cell holding part projecting from said body part toward the distal side of said living cell holding part; said body part is formed of a liquid nitrogen-resistant synthetic resin; said living cell holding part is formed of a liquid nitrogen-resistant synthetic resin; said living cell holding part is plate-shaped and extends in a longitudinal direction of said living cell cryopreservation tool; said living cell holding part has a plurality of living cell accommodation concaves provided on a surface of said plate-shaped living cell holding part; said plurality of living cell accommodation concaves are arranged in said longitudinal direction of said living cell cryopreservation tool; said living cell accommodation concaves are approximately hemispherical concaves; said living cell holding part has a groove communicating with said living cell accommodation concave and said living cell accommodation concave adjacent to each other; and said groove has cryopreservation liquid discharge passages in said living cell accommodation concave.",
    "2. The cell cryopreservation tool according to claim 1, wherein said living cell holding part is transparent or semitransparent.",
    "3. The cell cryopreservation tool according to claim 1, wherein said plurality of living cell accommodation concaves is linearly arranged on said living cell attaching and holding portion from a position spaced at a predetermined interval from a distal end of said cryopreservation tool toward a proximal side thereof.",
    "4. The living cell cryopreservation tool according to claim 1, wherein said living cell attaching and holding portion has bulged portions formed on a backside of each of the portions of said living cell attaching and holding portion.",
    "5. The living cell cryopreservation tool according to claim 1, wherein said living cell holding part has two side bulged portions which are formed at both sides of a portion of said living cell attaching and holding portion where said living cell accommodation concaves are formed and are extended in said longitudinal direction of said living cell attaching and holding portion.",
    "6. The living cell cryopreservation tool according to claim 1, wherein said living cell attaching and holding portion has a projected portion formed at a distal end side thereof than said living cell accommodation concave positioned nearer to said distal end thereof than said other living cell accommodation concaves.",
    "7. The living cell cryopreservation tool according to claim 1, wherein said living cell attaching and holding portion has a distal groove extended toward a distal end thereof from said living cell accommodation concave positioned nearer to said distal end thereof than any other living cell accommodation concaves.",
    "8. The living cell cryopreservation tool according to claim 1, wherein said living cell attaching and holding portion has a distal groove extending toward a distal end thereof from said living cell accommodation concave positioned nearer to said distal end thereof than any other living cell accommodation concaves and a projected portion formed at a distal end side thereof than said living cell accommodation concave positioned nearer to said distal end thereof than said other living cell accommodation concaves.",
    "9. The living cell cryopreservation tool according to claim 1, wherein said living cell attaching and holding portion has a proximal groove extended toward a proximal end thereof from said living cell accommodation concave portion positioned nearer to said proximal end thereof than any other living cell accommodation concaves.",
    "10. A living cell cryopreservation tool comprising: a body part and a living cell holding part projecting from said body part toward the distal side of said living cell holding part; and wherein said body part is formed of a liquid nitrogen-resistant synthetic resin; said living cell holding part is formed of a liquid nitrogen-resistant synthetic resin; said living cell holding part is plate-shaped and extends in a longitudinal direction of said living cell cryopreservation tool; said living cell holding part has a plurality of living cell accommodation concaves provided on a surface of said plate-shaped living cell holding part; said plurality of living cell accommodation concaves are arranged in said longitudinal direction of said living cell cryopreservation tool; said living cell accommodation concaves are approximately hemispherical concave portions; said living cell holding part having a groove communicating with said living cell accommodation concave and said living cell accommodation concave adjacent to each other; said living cell holding part having a distal groove extended toward a distal end thereof from said living cell accommodation concave positioned to a distal side than any other living cell accommodation concaves; said living cell holding part having a proximal groove extended toward a proximal end thereof from said living cell accommodation concave positioned to a proximal side than any other living cell accommodation concaves; and wherein said grooves having cryopreservation liquid discharge passages in said living cell accommodation concave.",
    "11. The living cell cryopreservation tool according to claim 10, wherein said living cell holding part has two side bulged portions which are formed at both sides of a portion of said living cell attaching and holding portion where said plurality of living cell accommodation concaves are formed and are extended in said longitudinal direction of said living cell attaching and holding portion.",
    "12. The living cell cryopreservation tool according to claim 10, wherein said liquid nitrogen-resistant synthetic resin is 3-polyethylene fluoride, low-density polyethylene, medium-density polyethylene, high-density polyethylene, polycarbonate, nylon, polysulfone, polyester, polystyrene, polyimide, ultra-high-molecular-weight polyethylene, ethylene-vinyl acetate copolymer or laminates of films formed of the synthetic resin.",
    "13. A living cell cryopreservation tool comprising: a body part and a living cell holding part projecting from said body part toward the distal side of said living cell holding member; wherein said body part is formed of a liquid nitrogen-resistant synthetic resin; said living cell holding part is formed of a liquid nitrogen-resistant synthetic resin; said living cell holding part is plate-shaped and extends in a longitudinal direction of said living cell cryopreservation tool; said living cell holding part has a plurality of living cell accommodation concaves provided on a surface of said plate-shaped living cell holding part; said plurality of living cell accommodation concaves are arranged in said longitudinal direction of said living cell cryopreservation tool; said living cell accommodation concaves are approximately hemispherical concave portions; said living cell holding part has a groove communicating with said living cell accommodation concave and said living cell accommodation concave adjacent to each other; said living cell holding part has a distal groove extended toward a distal end thereof from said living cell accommodation concave positioned to a distal side than any other living cell accommodation concaves; said living cell holding part has a proximal groove extended toward a proximal end thereof from said living cell accommodation concave positioned to a proximal side than any other living cell accommodation concaves; said grooves having cryopreservation liquid discharge passages in said living cell accommodation concave; and said living cell holding part has a projected portion formed at a distal end side thereof than said living cell accommodation concave positioned nearer to said distal end thereof than said other living cell accommodation concaves.",
    "14. The cell cryopreservation tool according to claim 13, wherein each of said plurality of living cell accommodation concaves is spaced at regular intervals.",
    "15. The living cell cryopreservation tool according to claim 13, wherein said liquid nitrogen-resistant synthetic resin is 3-polyethylene fluoride, low-density polyethylene, medium-density polyethylene, high-density polyethylene, polycarbonate, nylon, polysulfone, polyester, polystyrene, polyimide, ultra-high-molecular-weight polyethylene, ethylene-vinyl acetate copolymer or laminates of films formed of the synthetic resin."
  ],
  "cpc": [
    "C12M 45/22",
    "A01N 1/0257",
    "A01N 1/0268",
    "A01N 1/145",
    "A01N 1/147",
    "A61B 17/435"
  ],
  "assignees": [
    "KITAZATO BIOPHARMA CO LTD"
  ],
  "filing_date": "2012-10-01",
  "publication_date": "2017-01-10",
  "grant_date": "2017-01-10",
  "priority_date": "2011-10-03",
  "application_number": "US-201214349169-A",
  "family_id": "48043678",
  "citations": [
    "CN101087658A",
    "JP2000189155A",
    "JP2002315573A",
    "JP2004329202A",
    "JP2010148457A",
    "US2004259072A1",
    "US2008038155A1",
    "US2009123992A1",
    "US2009298116A1",
    "US2010151570A1",
    "US2010317108A1",
    "US2011129811A1",
    "US2011196358A1",
    "US2011207215A1",
    "US2011275153A1",
    "WO02085110A1",
    "WO2010047133A1"
  ]
}