Yeast cells having disrupted pathway from dihydroxyacetone phosphate to glycerol

{
  "publication_number": "US11691817B2",
  "country": "US",
  "kind": "B2",
  "title": "Yeast cells having disrupted pathway from dihydroxyacetone phosphate to glycerol",
  "abstract": "Yeast cells are genetically modified to disrupt a native metabolic pathway from dihydroxyacetone to glycerol. In certain aspects, the yeast cell is of the genera Kluyveromyces, Candida or Issatchenkia. In other aspects, the yeast cell is capable of producing at least one organic acid, such as lactate. The yeast cells produce significantly less glycerol than the wild-type strains, and usually produce greater yields of desired fermentation products. Yeast cells of the invention often grow well when cultivated, despite their curtailed glycerol production.",
  "claims": [
    "1. A yeast cell of species Issatchenkia orientalis that is genetically modified to produce lactate, said yeast cell further having a deletion or disruption of (i) a native gene encoding a glycerol-3-phosphate dehydrogenase and/or a native gene encoding a glycerol-3-phosphatase, and (ii) a pyruvate decarboxylase gene, wherein the yeast cell contains an exogenous lactate dehydrogenase gene.",
    "2. The yeast cell of claim 1 wherein the cell includes a deletion or disruption of at least one native glycerol-3-phosphate dehydrogenase gene.",
    "3. The yeast cell of claim 1 wherein the cell includes a deletion or disruption of at least one native glycerol-3-phosphatase gene.",
    "4. The yeast cell of claim 3 wherein the cell includes a deletion or disruption of at least one native glycerol-3-phosphase dehydrogenase gene and of at least one native glycerol-3-phosphatase gene.",
    "5. The yeast cell of claim 1, wherein the exogenous lactate dehydrogenase gene is obtained from an organism selected from the group consisting of L. helveticus, L. casei, B. megaterium, and P. acidilactici.",
    "6. The yeast cell of claim 1, wherein the strain is capable of producing at least 70 gram/liter lactate."
  ],
  "description_excerpt": "This application is a Continuation of U.S. patent application Ser. No. 15/941,274, filed 30 Mar. 2018 (now Granted as U.S Pat. No. 10,899,544), which is a Divisional of U.S. patent application Ser. No. 14/720,899, filed 25 May 2015 (now abandoned), which is a Divisional of U.S. patent application Ser. No. 12/281,286, filed 29 Aug. 2008 (now abandoned), which is a national phase application of International Application No. PCT/US2007/06408, filed 13 Mar. 2007 (now abandoned), which claims priority to United States Provisional Application No. 60/781,674, filed 13 Mar. 2006, each of which are hereby incorporated by reference in its entirety.\n\nThis invention was made under contract no. DE-FC36-03G013145 with the United States Department of Energy. The U.S. Government has certain rights to this invention.\n\nThis invention relates to certain genetically modified yeast, and fermentation processes to produce lactic acid using those genetically modified yeast.\n\nYeast are used as biocatalysts in a number of industrial fermentations. There is an increasing interest in using yeast to ferment sugars to organic acids such as lactic acid. As more organic acid is produced in these fermentations, the fermentation medium becomes increasingly acidic. Most bacteria that produce these organic acids do not perform well in strongly acidic environments - they either do not survive under those conditions or else produce so slowly that the process becomes economically unviable. As a result, it becomes necessary to buffer the medium to maintain a higher pH. This causes difficulty in recovering the product in acid form.",
  "cpc": [
    "B65G 15/12",
    "B65G 17/12",
    "C12N 15/52",
    "C12P 7/06",
    "C12P 7/40",
    "C12P 7/56",
    "E01B 29/32",
    "Y02E 50/10",
    "Y10T 137/85986"
  ],
  "ipc": [
    "B65G 15/12",
    "B65G 17/12",
    "C12N 15/52",
    "C12P 7/06",
    "C12P 7/40",
    "C12P 7/56",
    "E01B 29/32"
  ],
  "assignees": [
    "Cargill Inc"
  ],
  "inventors": [
    "Catherine Asleson Dundon",
    "Pirkko Suominen",
    "Aristos Aristidou",
    "Brian J. Rush",
    "Kari Koivuranta",
    "Benjamin Matthew Hause",
    "Thomas William McMullin",
    "Kevin Roberg-Perez"
  ],
  "filing_date": "2021-01-21",
  "publication_date": "2023-07-04",
  "grant_date": "2023-07-04",
  "priority_date": "2006-03-13",
  "application_number": "US-202117154190-A",
  "family_id": "38510074",
  "cited_by_count": 0,
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}