Patent · US5750338A · A · US

Target and background capture methods with amplification for affinity assays

(11) Publication number

US5750338A

(21) Application number

US-23808094-A

(22) Filing date

1994-05-03

(30) Priority date

1986-10-23

(43) Publication date

1998-05-12

(45) Date of grant

1998-05-12

(52) CPC

C12N Microorganisms or enzymes; compositions thereof; propagating, preserving, or maintaining microorganisms; mutation or genetic engineering; culture media: 15/1006
C12Q Measuring or testing processes involving enzymes, nucleic acids or microorganisms; compositions or test papers therefor; processes of preparing such compositions; condition-responsive control in microbiological or enzymological processes: 1/6813, 1/686
G01N Investigating or analysing materials by determining their chemical or physical properties: 35/0098

(73) Assignee

AMOCO CORP

(54) Title

Target and background capture methods with amplification for affinity assays

(57) Abstract

A method of assay for target polynucleotides includes steps of isolating target polynucleotides from extraneous non-target polynucleotides, debris, and impurities and amplifying the target polynucleotide.

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Claims (40)

A method for amplifying a target polynucleotide contained in a sample comprising the steps of: (a) contacting the sample with a first support which binds to the target polynucleotide; (b) substantially separating the support and bound target polynucleotide from the sample; and (c) amplifying the target polynucleotide.
The method of claim 1 wherein the first support is retrievable.
The method of claim 1 wherein the first support includes a probe which binds with the target polynucleotide.
The method of claim 1 wherein the target polynucleotide is amplified with a polymerase.
The method of claim 4 wherein the polymerase is a DNA polymerase, an RNA polymerase, a transcriptase or Qβ replicase.
The method of claim 4 wherein the target polynucleotide is a DNA polynucleotide and the polymerase is a DNA polymerase.
A method for detecting a target polynucleotide contained in a sample comprising the steps of: (a) contacting the sample with a first support which binds to the target polynucleotide; (b) substantially separating the first support and bound target polynucleotide from the sample; (c) amplifying the target polynucleotide; and (d) detecting the presence of the amplified target polynucleotide.
The method of claim 7 wherein the first support is retrievable.
The method of claim 8 wherein the first support includes a probe which binds with the target polynucleotide.
The method of claim 7 wherein the target polynucleotide is amplified with a polymerase.
The method of claim 10 wherein the polymerase is a DNA polymerase, an RNA polymerase, a transcriptase or Qβ replicase.
The method of claim 11 wherein the target polynucleotide is a DNA polynucleotide and the polymerase is a DNA polymerase.
The method of claim 7 wherein the amplified target polynucleotide is contacted with a label.
The method of claim 7 wherein the amplified target polynucleotide is contacted with a labeled probe.
The method of claim 7 wherein the amplified target polynucleotide is contacted with a second support which binds to the amplified target polynucleotide.
The method of claim 15 wherein the amplified target polynucleotide is contacted with a labeled probe.
The method of claim 16 wherein the target polynucleotide is amplified with a polymerase.
The method of claim 17 wherein the target polynucleotide is a DNA polynucleotide and the polymerase is a DNA polymerase.
A method for detecting a target polynucleotide contained in a sample comprising the steps of: (a) contacting the sample with a first support which binds to the target polynucleotide; (b) substantially separating the first support and bound target polynucleotide from the sample; (c) amplifying the sample with a DNA polymerase; (d) contacting the amplified target polynucleotide with a second support which binds to the amplified target polynucleotide and also with a labeled probe which binds to the amplified target polynucletide; and (e) detecting the presence of the amplified target polynucleotide.
A kit for detecting a target polynucleotide contained in a sample comprising: (a) means for substantially separating the target polynucleotide from the sample; (b) means for amplifying the target polynucleotide; (c) means for binding the amplified target polynucleotide to a solid support; and (d) means for labeling the amplified target polynucleotide.
The kit of claim 20 wherein: (a) the means for substantially separating the target polynucleotide from the sample include a first support; (b) the means for amplifying the target polynucleotide include a polymerase; (c) the means for binding that amplified target polynucleotide to a solid support include a capture probe which binds to the solid support and to the amplified target polynucleotide; and (d) a detector probe for labeling the amplified target polynucleotide.
The kit of claim 21 further comprising a capture probe which binds to the first support and to the target.
The kit of claim 22 wherein the polymerase is a DNA polymerase and the detector probe is labeled.
A kit for amplifying a target polynucleotide contained in a sample comprising: (a) means for substantially separating the target polynucleotide from the sample and (b) means for amplifying the target polynucleotide.
The kit of claim 24 wherein: (a) the means for substantially separating the target polynucleotide from the sample includes a support which binds to the target polynucleotide and (b) the means for amplifying the target polynucleotide includes a polymerase.
The kit of claim 25 wherein: (a) the polymerase is a DNA polymerase; and (b) the means for substantially separating the target polynucleotide from the sample includes a probe which binds to the target polynucleotide and the support.
A method for amplifying a target polynucleotide contained in a sample medium comprising the steps of: (a) contacting the sample medium with reagent comprising a first nucleic acid probe which binds to the target to form a probe-target complex; (b) contacting the sample medium with a support which binds to the first nucleic acid probe of the probe-target complex; (c) substantially separating the support and bound probe-target complex from the sample medium; (d) contacting the support and bound probe-target complex with a second medium; (e) releasing the probe-target complex into the second medium; (f) substantially separating the support from the second medium; and (g) amplifying the target polynucleotide.
A method for detecting a target polynucleotide contained in a sample medium comprising the steps of: (a) contacting the sample medium with reagent comprising a first nucleic acid probe which binds to the target to form a probe-target complex; (b) contacting the sample medium with a support which binds to the first nucleic acid probe of the probe-target complex; (c) substantially separating the support and bound probe-target complex from the sample medium; (d) contacting the support and bound probe-target complex with a second medium; (e) releasing the probe-target complex into the second medium; (f) substantially separating the support from the second medium; (g) amplifying the target polynucleotide; and (h) detecting the presence of the target polynucleotide.
The method of detecting a target polynucleotide of claim 28 wherein the target polynucleotide is amplified with a polymerase.
The method for detecting a target polynucleotide of claim 29 wherein the polymerase is a DNA polymerase, an RNA polymerase, a transcriptase, or Qβ replicase.
The method for detecting a target polynucleotide of claim 30 wherein the polymerase is a DNA polymerase.
The method for amplifying a target polynucleotide of claim 27 wherein the target polynucleotide is amplified with a polymerase.
The method for amplifying a target polynucleotide of claim 32 wherein the polymerase is a DNA polymerase.
A method for amplifying a target polynucleotide contained in a sample medium comprising the steps of: (a) contacting the sample medium with a support and a probe which binds to the target polynucleotide and the support; (b) substantially separating the support and bound probe and target polynucleotide from the sample medium; (c) contacting the support and bound probe and target polynucleotide with a second medium; (d) releasing the target polynucleotide into the second medium; (e) substantially separating the support and bound probe from the second medium; and (f) amplifying the target polynucleotide.
The method for amplifying a target polynucleotide of claim 34 wherein the target polynucleotide is amplified a polymerase.
The method for amplifying a target polynucleotide of claim 35 wherein the polymerase is a DNA polymerase, an RNA polymerase, a transcriptase or Qβ replicase.
The method for amplifying a target polynucleotide of claim 36 wherein the polymerase is a DNA polymerase.
A method for detecting a target polynucleotide contained in a sample medium comprising the steps of: (a) contacting the sample medium with a support and probe which binds to the target polynucleotide and the support; (b) substantially separating the support and bound probe and target polynucleotide from the sample medium; (c) contacting the support and bound probe and target polynucleotide with a second medium; (d) releasing the target polynucleotide into the second medium; (e) substantially separating the support and bound probe form the second medium; (f) amplifying the target polynucleotide; and (g) detecting the presence of the amplified target polynucleotide.
The method for detecting a target polynucleotide of claim 38 wherein the target polynucleotide is amplified with a polymerase.
The method for detecting a target polynucleotide of claim 39 wherein the polymerase is a DNA polymerase.

Citations (5)

EP0139489A2
EP0159719B1
US4851331A
US5200314A
US5232829A

Record as JSON

{
  "publication_number": "US5750338A",
  "country": "US",
  "kind": "A",
  "title": "Target and background capture methods with amplification for affinity assays",
  "abstract": "A method of assay for target polynucleotides includes steps of isolating target polynucleotides from extraneous non-target polynucleotides, debris, and impurities and amplifying the target polynucleotide.",
  "claims": [
    "1. A method for amplifying a target polynucleotide contained in a sample comprising the steps of: (a) contacting the sample with a first support which binds to the target polynucleotide; (b) substantially separating the support and bound target polynucleotide from the sample; and (c) amplifying the target polynucleotide.",
    "2. The method of claim 1 wherein the first support is retrievable.",
    "3. The method of claim 1 wherein the first support includes a probe which binds with the target polynucleotide.",
    "4. The method of claim 1 wherein the target polynucleotide is amplified with a polymerase.",
    "5. The method of claim 4 wherein the polymerase is a DNA polymerase, an RNA polymerase, a transcriptase or Qβ replicase.",
    "6. The method of claim 4 wherein the target polynucleotide is a DNA polynucleotide and the polymerase is a DNA polymerase.",
    "7. A method for detecting a target polynucleotide contained in a sample comprising the steps of: (a) contacting the sample with a first support which binds to the target polynucleotide; (b) substantially separating the first support and bound target polynucleotide from the sample; (c) amplifying the target polynucleotide; and (d) detecting the presence of the amplified target polynucleotide.",
    "8. The method of claim 7 wherein the first support is retrievable.",
    "9. The method of claim 8 wherein the first support includes a probe which binds with the target polynucleotide.",
    "10. The method of claim 7 wherein the target polynucleotide is amplified with a polymerase.",
    "11. The method of claim 10 wherein the polymerase is a DNA polymerase, an RNA polymerase, a transcriptase or Qβ replicase.",
    "12. The method of claim 11 wherein the target polynucleotide is a DNA polynucleotide and the polymerase is a DNA polymerase.",
    "13. The method of claim 7 wherein the amplified target polynucleotide is contacted with a label.",
    "14. The method of claim 7 wherein the amplified target polynucleotide is contacted with a labeled probe.",
    "15. The method of claim 7 wherein the amplified target polynucleotide is contacted with a second support which binds to the amplified target polynucleotide.",
    "16. The method of claim 15 wherein the amplified target polynucleotide is contacted with a labeled probe.",
    "17. The method of claim 16 wherein the target polynucleotide is amplified with a polymerase.",
    "18. The method of claim 17 wherein the target polynucleotide is a DNA polynucleotide and the polymerase is a DNA polymerase.",
    "19. A method for detecting a target polynucleotide contained in a sample comprising the steps of: (a) contacting the sample with a first support which binds to the target polynucleotide; (b) substantially separating the first support and bound target polynucleotide from the sample; (c) amplifying the sample with a DNA polymerase; (d) contacting the amplified target polynucleotide with a second support which binds to the amplified target polynucleotide and also with a labeled probe which binds to the amplified target polynucletide; and (e) detecting the presence of the amplified target polynucleotide.",
    "20. A kit for detecting a target polynucleotide contained in a sample comprising: (a) means for substantially separating the target polynucleotide from the sample; (b) means for amplifying the target polynucleotide; (c) means for binding the amplified target polynucleotide to a solid support; and (d) means for labeling the amplified target polynucleotide.",
    "21. The kit of claim 20 wherein: (a) the means for substantially separating the target polynucleotide from the sample include a first support; (b) the means for amplifying the target polynucleotide include a polymerase; (c) the means for binding that amplified target polynucleotide to a solid support include a capture probe which binds to the solid support and to the amplified target polynucleotide; and (d) a detector probe for labeling the amplified target polynucleotide.",
    "22. The kit of claim 21 further comprising a capture probe which binds to the first support and to the target.",
    "23. The kit of claim 22 wherein the polymerase is a DNA polymerase and the detector probe is labeled.",
    "24. A kit for amplifying a target polynucleotide contained in a sample comprising: (a) means for substantially separating the target polynucleotide from the sample and (b) means for amplifying the target polynucleotide.",
    "25. The kit of claim 24 wherein: (a) the means for substantially separating the target polynucleotide from the sample includes a support which binds to the target polynucleotide and (b) the means for amplifying the target polynucleotide includes a polymerase.",
    "26. The kit of claim 25 wherein: (a) the polymerase is a DNA polymerase; and (b) the means for substantially separating the target polynucleotide from the sample includes a probe which binds to the target polynucleotide and the support.",
    "27. A method for amplifying a target polynucleotide contained in a sample medium comprising the steps of: (a) contacting the sample medium with reagent comprising a first nucleic acid probe which binds to the target to form a probe-target complex; (b) contacting the sample medium with a support which binds to the first nucleic acid probe of the probe-target complex; (c) substantially separating the support and bound probe-target complex from the sample medium; (d) contacting the support and bound probe-target complex with a second medium; (e) releasing the probe-target complex into the second medium; (f) substantially separating the support from the second medium; and (g) amplifying the target polynucleotide.",
    "28. A method for detecting a target polynucleotide contained in a sample medium comprising the steps of: (a) contacting the sample medium with reagent comprising a first nucleic acid probe which binds to the target to form a probe-target complex; (b) contacting the sample medium with a support which binds to the first nucleic acid probe of the probe-target complex; (c) substantially separating the support and bound probe-target complex from the sample medium; (d) contacting the support and bound probe-target complex with a second medium; (e) releasing the probe-target complex into the second medium; (f) substantially separating the support from the second medium; (g) amplifying the target polynucleotide; and (h) detecting the presence of the target polynucleotide.",
    "29. The method of detecting a target polynucleotide of claim 28 wherein the target polynucleotide is amplified with a polymerase.",
    "30. The method for detecting a target polynucleotide of claim 29 wherein the polymerase is a DNA polymerase, an RNA polymerase, a transcriptase, or Qβ replicase.",
    "31. The method for detecting a target polynucleotide of claim 30 wherein the polymerase is a DNA polymerase.",
    "32. The method for amplifying a target polynucleotide of claim 27 wherein the target polynucleotide is amplified with a polymerase.",
    "33. The method for amplifying a target polynucleotide of claim 32 wherein the polymerase is a DNA polymerase.",
    "34. A method for amplifying a target polynucleotide contained in a sample medium comprising the steps of: (a) contacting the sample medium with a support and a probe which binds to the target polynucleotide and the support; (b) substantially separating the support and bound probe and target polynucleotide from the sample medium; (c) contacting the support and bound probe and target polynucleotide with a second medium; (d) releasing the target polynucleotide into the second medium; (e) substantially separating the support and bound probe from the second medium; and (f) amplifying the target polynucleotide.",
    "35. The method for amplifying a target polynucleotide of claim 34 wherein the target polynucleotide is amplified a polymerase.",
    "36. The method for amplifying a target polynucleotide of claim 35 wherein the polymerase is a DNA polymerase, an RNA polymerase, a transcriptase or Qβ replicase.",
    "37. The method for amplifying a target polynucleotide of claim 36 wherein the polymerase is a DNA polymerase.",
    "38. A method for detecting a target polynucleotide contained in a sample medium comprising the steps of: (a) contacting the sample medium with a support and probe which binds to the target polynucleotide and the support; (b) substantially separating the support and bound probe and target polynucleotide from the sample medium; (c) contacting the support and bound probe and target polynucleotide with a second medium; (d) releasing the target polynucleotide into the second medium; (e) substantially separating the support and bound probe form the second medium; (f) amplifying the target polynucleotide; and (g) detecting the presence of the amplified target polynucleotide.",
    "39. The method for detecting a target polynucleotide of claim 38 wherein the target polynucleotide is amplified with a polymerase.",
    "40. The method for detecting a target polynucleotide of claim 39 wherein the polymerase is a DNA polymerase."
  ],
  "cpc": [
    "C12N 15/1006",
    "C12Q 1/6813",
    "C12Q 1/686",
    "G01N 35/0098"
  ],
  "assignees": [
    "AMOCO CORP"
  ],
  "filing_date": "1994-05-03",
  "publication_date": "1998-05-12",
  "grant_date": "1998-05-12",
  "priority_date": "1986-10-23",
  "application_number": "US-23808094-A",
  "family_id": "32854605",
  "citations": [
    "EP0139489A2",
    "EP0159719B1",
    "US4851331A",
    "US5200314A",
    "US5232829A"
  ]
}

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