Bacterial artificial chromosome

{
  "id": "MLC-T-ENV-002661",
  "term": "Bacterial artificial chromosome",
  "field": "Environment",
  "term_source": "Bacterial Artificial Chromosome",
  "definition": "1. A vector used to clone DNA fragments (100- to 300-kb insert size; average, 150 kb) in Escherichia coli cells. Based on naturally occurring F-factor plasmid found in the bacterium E. coli.\n\n2. DNA constructs that are composed of, at least, a replication origin, for successful replication, propagation to and maintenance as an extra chromosome in bacteria. In addition, they can carry large amounts (about 200 kilobases) of other sequence for a variety of bioengineering purposes.\n\n3. A bacterial artificial chromosome (BAC) is an engineered DNA molecule used to clone DNA sequences in bacterial cells (for example, E. coli). BACs are often used in connection with DNA sequencing. Segments of an organism's DNA, ranging from 100,000 to about 300,000 base pairs, can be inserted into BACs.",
  "abbreviation": "BAC",
  "subject": "Biological substances",
  "references": [
    "DOE Human Genome Project Information Genome Glossary",
    "NIH Genetics Home Reference Glossary"
  ],
  "url": "https://mlchart.com/terminology/environment/bacterial-artificial-chromosome/"
}