Method for purification of nucleic acids, particularly from fixed tissue

{
  "publication_number": "US2014186236A1",
  "country": "US",
  "kind": "A1",
  "title": "Method for purification of nucleic acids, particularly from fixed tissue",
  "abstract": "The invention relates to a method for purification of nucleic acids, to a kit for performing the method according to the invention and to a new application of magnetic particles for purification of a biological sample. The method according to the invention comprises the following steps: a) accommodating of the sample in a first sample vessel in an aqueous solution and lysing of the sample under non-chaotropic conditions; suspending of first magnetic particles in the solution and inserting of the first sample vessel in a sample vessel holder, wherein the sample vessel is inserted in the annular interior space of a ring magnet associated with the sample vessel holder; separating of the solution from the magnetic particles; and isolating of the nucleic acids from the solution.",
  "claims": [
    "1. An automated system comprising: a rack for at least one sample vessel; a temperature-controlled device having at least one slot for the sample vessel; a sample vessel holder for accommodating at least one sample vessel; a device for transporting the sample vessel holder from the rack to the temperature-controlled device; a device for transferring liquid from a sample vessel and/or into a sample vessel; a control for controlling the transport device, the liquid transfer device, and for controlling the temperature of the temperature-controlled device; wherein the sample vessel holder for accommodating the at least one sample vessel comprises at least one annular magnet, in the annular interior space of which the sample vessel can be accommodated. 2. A sample vessel holder for accommodating the at least one sample vessel, comprising at least one annular magnet, in the annular interior space of which the sample vessel can be accommodated. 3. The sample vessel holder as claimed in claim 2, wherein the ring inner diameter of the annular magnet is, at its narrowest point, 4 to 20 mm, preferably 6-12, in particular 8 mm."
  ],
  "description_excerpt": "This application is a divisional of U.S. application Ser. No. 13/133,182 which was filed on Jun. 7, 2011 which is the US National Stage of International Application No. PCT/EP2009/065534 filed Nov. 20, 2009. The International Application claims the benefit of German Patent Office Application No. 102008061714.8 filed Dec. 12, 2008. All of the applications are incorporated by reference herein in their entirety.\n\nThe invention relates to a method for purifying nucleic acids, to a system for carrying out the method according to the invention, and also to a sample vessel holder for the purification of a biological sample.\n\nMolecular diagnostics has recently become increasingly important. It has found a way into the clinical diagnosis of diseases (inter alia, detection of infectious agents, detection of mutations of the genome, discovery of circulating tumor cells, and identification of risk factors for the predisposition to a disease). But also in veterinary medicine, environmental analysis, and food testing, use is meanwhile being made of molecular diagnostics methods. Tests at pathology/cytology institutes or in the context of forensic problems represent a further area of application. But also in the context of healthcare (e.g., tests on blood supplies for absence of infectious agents), use is meanwhile being made of gene diagnostics, and lawmakers are planning to regulate such tests by law in the future.",
  "cpc": [
    "C12Q 1/6806",
    "C07B 63/00",
    "C12N 15/1013",
    "Y10T 137/8376"
  ],
  "ipc": [
    "C12Q 1/68",
    "C07B 63/00",
    "C12N 15/10",
    "G01N 33/553"
  ],
  "assignees": [
    "Siemens Healthcare Diagnostics Inc"
  ],
  "inventors": [
    "Heike Euting",
    "Guido Hennig",
    "Alexandre Izmailov"
  ],
  "filing_date": "2014-03-06",
  "publication_date": "2014-07-03",
  "priority_date": "2008-12-12",
  "application_number": "US-201414199019-A",
  "family_id": "41683033",
  "cited_by_count": 13,
  "citations": [
    "US5897783A"
  ]
}