Lymphocyte chemoattractant factor and uses thereof

{
  "publication_number": "US5766866A",
  "country": "US",
  "kind": "A",
  "title": "Lymphocyte chemoattractant factor and uses thereof",
  "abstract": "Disclosed is a substantially pure antibody which specifically binds a LCF polypeptide and methods of using such antibodies.",
  "claims": [
    "1. A lymphocyte chemoattractant factor (LCF) immunoassay kit comprising an antibody which binds a LCF polypeptide having SEQ ID NO. 1.",
    "2. The lymphocyte chemoattractant factor immunoassay kit of claim 1, further comprising means for detecting binding of said antibody to said lymphocyte chemoattractant factor polypeptide.",
    "3. A method of detecting lymphocyte chemoattractant factor (LCF) in a biological sample, said method comprising: (a) contacting said biological sample with an antibody which binds to lymphocyte chemoattractant factor having SEQ ID NO. 1; and (b) detecting immune complex formation, between said antibody and a sample constituent, as indicative of the presence of said lymphocyte chemoattractant factor in said sample.",
    "4. The method of claim 3, wherein said immune complex formation is detected by ELISA.",
    "5. The method of claim 3, wherein said immune complex formation is detected by Western blot analysis."
  ],
  "description_excerpt": "This work was supported in part by a grant from the Federal Government and the Government therefore has certain rights in the invention.\n\nThis is a divisional of application Ser. No. 08/480,156, filed on Jun. 7, 1995; which is a continuation-in-part of U.S. Ser. No. 08/354,961, filed Dec. 13, 1994; which is a continuation of U.S. Ser. No. 08/068,949, filed May 21, 1993 now abandoned.\n\nThis invention relates to recombinant LCF, DNA, and uses thereof.\n\nCD4, a cell-cell adhesion protein, is expressed on a subset of T lymphocytes (Krensky et al., Proc. Natl. Acad. Sci. U.S.A. 79:2365-2369, 1982; Biddison et al., J. Exp. Med. 156:1065-1076, 1982; and Wilde et al., J. Immunol. 131:152-157, 1983), mononuclear cells (Stewart et al., J. Immunol. 136:3773-3778, 1986), and eosinophils (Rand et al., J. Exp. Med. 173:1521-1528, 1991). In lymphocytes, CD4 contributes to antigen receptor signaling (Collins et al., J. Immunol. 148:2159-2162, 1992; Anderson et al., J. Immunol. 139-678-682, 1987; Eichmann et al., J. Immunol. 17:643-650, 1987; Walker et al., Eur. J. Immunol. 17:873-880 1987; and Sleckman et al., Nature 328:351-353, 1987) by direct interaction with MHC Class II molecules (Doyle et al., Nature 330:256-259, 1987). In addition, a natural soluble lymphokine, lymphocyte chemoattractant factor (LCF), requires cell surface expression of CD4 to induce chemotactic activity in monocytes (Cruikshank et al., J. Exp. Med. 173:1521-1528, 1991) and T lymphocytes (Cruikshank et al., J. Immunol. 138:3817-3823, 1987; Cruikshank et al., J. Immunol. 146:2928-2934, 1991).",
  "cpc": [
    "C07K 16/244",
    "A61K 38/2013",
    "C07K 14/5446",
    "C07K 16/22",
    "C07K 16/24",
    "C07K 2317/73",
    "Y10S 435/974",
    "Y10S 435/975"
  ],
  "ipc": [
    "A61K 38/20",
    "C07K 14/54",
    "C07K 16/22",
    "C07K 16/24"
  ],
  "assignees": [
    "Research Corp Technologies Inc"
  ],
  "inventors": [
    "David M. Center",
    "William W. Cruikshank",
    "Hardy Kornfeld"
  ],
  "filing_date": "1995-12-29",
  "publication_date": "1998-06-16",
  "grant_date": "1998-06-16",
  "priority_date": "1993-05-21",
  "application_number": "US-58068095-A",
  "family_id": "23906878",
  "cited_by_count": 34,
  "citations": [
    "US4486530A",
    "US4745051A",
    "WO1994028134A1"
  ]
}