Cloning and sequencing of allergens of Dermatophagoides (house dust mite)

{
  "publication_number": "US6060057A",
  "country": "US",
  "kind": "A",
  "title": "Cloning and sequencing of allergens of Dermatophagoides (house dust mite)",
  "abstract": "Isolated DNA encoding allergens of Dermatophagoides (house dust mites) particularly of the species Dermatophagoides farinae and Dermatophagoides pteronyssinus, which are protein allergens or peptides which include at least one epitope of the protein allergen. In particular, DNA encoding two major D. farinae allergens, Der f I and Der f II and DNA encoding a D. pteronyssinus allergen, Der p I. In addition, the proteins or peptides encoded by the isolated DNA, their use as diagnostic and therapeutic reagents and methods of diagnosing and treating sensitivity to house dust mite allergens.",
  "claims": [
    "1. An isolated recombinant protein allergen of Dermatophagoides farinae, said protein allergen selected from the group consisting of a Der f I protein allergen comprising the amino acid sequence shown in FIG. 2, or the mature portion thereof, and a Der f II protein allergen comprising the amino acid sequence shown in FIG. 6, or the mature portion thereof.",
    "2. An isolated protein allergen of claim 1, wherein the protein allergen is Der f I.",
    "3. An isolated protein allergen of claim 2 encoded by the a nucleotide sequence shown in FIG. 2.",
    "4. An isolated protein allergen of claim 2 encoded by the coding region of the nucleotide sequence shown in FIG. 2.",
    "5. An isolated protein allergen of claim 4 wherein the coding region consists of nucleotides 42-1004 of the nucleotide sequence shown in FIG. 2.",
    "6. A therapeutic composition comprising an isolated protein of claim 2 and a pharmaceutically acceptable carrier.",
    "7. A diagnostic reagent comprising a protein allergen of claim 2.",
    "8. An isolated protein allergen of claim 1, wherein the protein allergen is Der f II.",
    "9. An isolated protein of claim 8 encoded by the nucleotide sequence shown in FIG. 6.",
    "10. An isolated protein allergen of claim 8 encoded by the coding region of the nucleotide sequence shown in FIG. 6.",
    "11. An isolated protein allergen of claim 10 wherein the coding region consists of nucleotides 1-387 of the nucleotide sequence shown in FIG. 6.",
    "12. A therapeutic composition comprising an isolated protein of claim 8 and a pharmaceutically acceptable carrier.",
    "13. A diagnostic reagent comprising a protein allergen of claim 8.",
    "14. An isolated protein allergen of claim 1 wherein the mature portion consists of amino acid residues 1-223 of the amino acid sequence shown in FIG. 2.",
    "15. An isolated protein allergen of claim 1 wherein the mature portion consists of amino acid residues 1-129 of the amino acid sequence shown in FIG. 6.",
    "16. A therapeutic composition comprising an isolated protein of claim 1 and a pharmaceutically acceptable carrier.",
    "17. A diagnostic reagent comprising a protein allergen of claim 1."
  ],
  "description_excerpt": "This application is a continuation application of Ser. No. 08/301,137 filed on Sep. 6, 1994, pending, which in turn is a continuation application of Ser. No. 08/107,332 filed on Aug. 16, 1993, abanonded, which in turn is a continuation application of Ser. No. 07/580,655 filed on Sep. 11, 1990, abandoned, which is a continuation-in-part of Ser. No. 07/458,642 filed on Feb. 13, 1990, now abandoned. The contents of all of the aforementioned application(s) are hereby incorporated by reference.\n\nWork described herein was funded by grants from the Princess Margaret Children's Medical Research Foundation, the Australian Health and Medical Research Council and the Asthma Foundation of Australia.\n\nRecent reports have documented the importance of responses to the Group I and Group II allergens in house dust mite allergy. For example, it has been documented that over 60% of patients have at least 50% of their anti-mite antibodies directed towards these proteins (Lind, P. et al., Allergy. 39:259-274 (1984); van der Zee, J. S. et al., J. Allergy Clin. Immunol., 81:884-896 (1988)). It is possible that children show a greater degree of reactivity (Thompson, P. J. et al., Immunology, 64:311-314 (1988)). Allergy to mites of the genus Dermatophagoides (D.) is associated with conditions such as asthma, rhinitis and ectopic dermatitis. Two species, D. pteronyssinus and D. farinae, predominate and, as a result, considerable effort has been expended in trying to identify the allergens produced by these two species. D. pteronyssinus mites are the most common Dermatophagoides species in house dust in Western Europe and Australia. The species D.",
  "cpc": [
    "C07K 14/43531",
    "A61K 38/00",
    "A61P 37/08",
    "C07K 14/5403",
    "C12N 15/11"
  ],
  "ipc": [
    "A61K 39/35",
    "A61K 39/36",
    "A61K 49/00",
    "A61P 37/08",
    "C07H 21/04",
    "C07K 14/00",
    "C07K 14/435",
    "C07K 14/54",
    "C12N 15/09",
    "C12N 15/12",
    "C12P 21/02",
    "G01N 33/53",
    "G01N 33/569"
  ],
  "assignees": [
    "Immulogic Pharmaceutical Corp"
  ],
  "inventors": [
    "Wayne Robert Thomas",
    "Kaw-Yan Chua"
  ],
  "filing_date": "1995-06-05",
  "publication_date": "2000-05-09",
  "grant_date": "2000-05-09",
  "priority_date": "1990-02-13",
  "application_number": "US-46091895-A",
  "family_id": "24321976",
  "cited_by_count": 10,
  "citations": [
    "AU3132771A",
    "WO1988010297A1"
  ]
}