Internal Positive Control

{
  "id": "MLC-T-ENV-014770",
  "term": "Internal Positive Control",
  "field": "Environment",
  "definition": "1. The internal positive controls of known concentrations consists of a simple DNA template material for the assay target added to its own dedicated PCR reaction run in parallel with the samples on a test. A positive test result in this reaction validates the function not only of the shared master mix components tested by an internal control, but also uniquely shows the function of target-specific primers and probes. These controls might form the basis for a standard curve for quantitative assays by allowing the quantitation standard to take into direct account possible variations in extraction efficiency across a range of target concentrations.\n\n2. Internal positive controls of known concentrations consists of a simple DNA template material for the assay target added to its own dedicated PCR reaction run in parallel with the samples on a test. A positive test result in this reaction validates the function not only of the shared master mix components tested by an internal control, but also uniquely shows the function of target-specific primers and probes. These controls might form the basis for a standard curve for quantitative assays by allowing the quantitation standard to take into direct account possible variations in extraction efficiency across a range of target concentrations. [EPA 815-B-04-001]",
  "subject": "Science and research",
  "references": [
    "EPA 815-B-04-001",
    "Environmental Sampling and Analytical Methods (ESAM) Program Glossary"
  ],
  "url": "https://mlchart.com/terminology/environment/internal-positive-control/"
}