Shotgun Sequencing

{
  "id": "MLC-T-ENV-025497",
  "term": "Shotgun Sequencing",
  "field": "Environment",
  "definition": "1. Sequencing method that involves randomly sequenced cloned pieces of the genome, with no foreknowledge of where the piece originally came from. This can be contrasted with \"directed\" strategies, in which pieces of DNA from known chromosomal locations are sequenced. Because there are advantages to both strategies, researchers use both random (or shotgun) and directed strategies in combination to sequence the human genome.\n\n2. Shotgun sequencing is a laboratory technique for determining the DNA sequence of an organism's genome. The method involves breaking the genome into a collection of small DNA fragments that are sequenced individually. A computer program looks for overlaps in the DNA sequences and uses them to place the individual fragments in their correct order to reconstitute the genome.\n\n3. a DNA sequencing technique in which a large number of small fragments of a long DNA strand are generated at random, sequenced, and computationally reassembled to form a sequence of the original strand; also known as \"shotgun cloning\"",
  "references": [
    "NIH Genetics Home Reference Glossary",
    "NOAA Coral Reef Glossary"
  ],
  "url": "https://mlchart.com/terminology/environment/shotgun-sequencing/"
}