Fluorescence in situ Hybridization

{
  "id": "MLC-T-ENV-011365",
  "term": "Fluorescence in situ Hybridization",
  "field": "Environment",
  "definition": "1. Hybridization of cloned DNA to intact chromosomes, where the cloned DNA has been labelled with a fluorescent dye. This is the major method of physical mapping of cloned DNA fragments on chromosomes.\n\n2. Fluorescence in situ hybridization (FISH) is a laboratory technique for detecting and locating a specific DNA sequence on a chromosome. The technique relies on exposing chromosomes to a small DNA sequence called a probe that has a fluorescent molecule attached to it. The probe sequence binds to its corresponding sequence on the chromosome.\n\n3. A physical mapping approach that uses fluorescein tags to detect hybridization of probes with metaphase chromosomes and with the less-condensed somatic interphase chromatin.\n\n4. A type of in situ hybridization in which target sequences are stained with fluorescent dye so their location and size can be determined using fluorescence microscopy. This staining is sufficiently distinct that the hybridization signal can be seen both in metaphase spreads and in interphase nuclei.",
  "subject": "Research",
  "references": [
    "NOAA Coral Reef Glossary",
    "NIH Genetics Home Reference Glossary",
    "NLM Medical Subject Headings (MeSH)"
  ],
  "url": "https://mlchart.com/terminology/environment/fluorescence-in-situ-hybridization/"
}