Northern blot

{
  "id": "MLC-T-ENV-019111",
  "term": "Northern blot",
  "field": "Environment",
  "term_source": "Northern Blot",
  "definition": "1. Northern blot is a laboratory technique used to detect a specific RNA sequence in a blood or tissue sample. The sample RNA molecules are separated by size using gel electrophoresis. The RNA fragments are transferred out of the gel to the surface of a membrane. The membrane is exposed to a DNA probe labeled with a radioactive or chemical tag. If the probe binds to the membrane, then the complementary RNA sequence is present in the sample.\n\n2. A gel-based laboratory procedure that locates mRNA sequences on a gel that are complementary to a piece of DNA used as a probe.\n\n3. A technique used to separate and identify pieces of RNA.",
  "subject": "Research",
  "references": [
    "NIH Genetics Home Reference Glossary",
    "DOE, Oak Ridge National Laboratory, Human Genome Project Information, Genome Glossary",
    "EPA EV-Research-Research Resources-Dedicated Science Technologies & Equipment"
  ],
  "url": "https://mlchart.com/terminology/environment/northern-blot/"
}