In situ hybridization

{
  "id": "MLC-T-ENV-013959",
  "term": "In situ hybridization",
  "field": "Environment",
  "term_source": "In Situ Hybridization",
  "definition": "1. A technique that localizes specific nucleic acid sequences within intact chromosomes, eukaryotic cells, or bacterial cells through the use of specific nucleic acid-labeled probes.\n\n2. A method of detecting the presence of specific nucleic acid sequences within a cytological preparation. A DNA or RNA probe is labeled, radioactively or chemically, and hybridized to a cytological preparation to detect RNA, or to a denatured cytological preparation to detect complementary DNA. The hybridization is detected by autoradiography (for radioactive probes) or by chromogenic reactions or fluorescence (for chemically-labeled probes).",
  "subject": "Research",
  "references": [
    "NIH Medical Subject Headings (MeSH)",
    "NOAA Coral Reef Glossary"
  ],
  "url": "https://mlchart.com/terminology/environment/in-situ-hybridization/"
}