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Northern blot

Term · Chemistry · MLC-T-CHM-008083

A molecular biology technique used to detect specific RNA sequences in a sample. RNA is first separated by gel electrophoresis, then transferred to a membrane, and finally hybridized with a labeled probe complementary to the target RNA. This method provides information about gene expression levels by indicating the presence and abundance of particular RNA molecules.

Table 1. Record
IdentifierMLC-T-CHM-008083
FieldChemistry
SubjectAnalytical Chemistry
ReferencesPAC, 2018, 90, 1121. 'Terminology of bioanalytical methods (IUPAC Recommendations 2018)' on page 1147 (https://doi.org/10.1515/pac-2016-1120)
See alsoSouthern blot; Western blot; Gene expression
Record as JSON
{
  "id": "MLC-T-CHM-008083",
  "term": "Northern blot",
  "field": "Chemistry",
  "definition": "A molecular biology technique used to detect specific RNA sequences in a sample. RNA is first separated by gel electrophoresis, then transferred to a membrane, and finally hybridized with a labeled probe complementary to the target RNA. This method provides information about gene expression levels by indicating the presence and abundance of particular RNA molecules.",
  "subject": "Analytical Chemistry",
  "see_also": [
    "Southern blot",
    "Western blot",
    "gene expression"
  ],
  "references": [
    "PAC, 2018, 90, 1121. 'Terminology of bioanalytical methods (IUPAC Recommendations 2018)' on page 1147 (https://doi.org/10.1515/pac-2016-1120)"
  ],
  "url": "https://mlchart.com/terminology/chemistry/northern-blot/"
}

Record 8,582 of 13,678 in Chemistry terminology (MLC-0109). Request the full dataset.