Western blot

{
  "id": "MLC-T-CHM-012679",
  "term": "Western blot",
  "field": "Chemistry",
  "definition": "A laboratory method for identifying particular proteins within a complex biological sample. This technique separates proteins by size using gel electrophoresis, then transfers them to a membrane where specific antibodies bind to the target protein for detection. The presence of the protein is typically visualized through chemiluminescence or colorimetric reactions.",
  "subject": "Analytical Chemistry",
  "see_also": [
    "Northern blot",
    "Southern blot"
  ],
  "references": [
    "PAC, 2018, 90, 1121. 'Terminology of bioanalytical methods (IUPAC Recommendations 2018)' on page 1165 (https://doi.org/10.1515/pac-2016-1120)"
  ],
  "url": "https://mlchart.com/terminology/chemistry/western-blot/"
}