Western Blot

{
  "id": "MLC-T-ENV-030326",
  "term": "Western Blot",
  "field": "Environment",
  "definition": "1. Western blotting is a laboratory technique used to detect a specific protein in a blood or tissue sample. The method involves using gel electrophoresis to separate the sample's proteins. The separated proteins are transferred out of the gel to the surface of a membrane. The membrane is exposed to an antibody specific to the target protein. Binding of the antibody is detected using a radioactive or chemical tag. A western blot is sometimes used to diagnose disease.\n\n2. A technique used to identify and locate proteins based on their ability to bind to specific antibodies.",
  "references": [
    "NIH Genetics Home Reference Glossary",
    "DOE Oak Ridge National Laboratory, Human Genome Project Information, Genome Glossary"
  ],
  "url": "https://mlchart.com/terminology/environment/western-blot/"
}