Southern blot

{
  "id": "MLC-T-ENV-026229",
  "term": "Southern blot",
  "field": "Environment",
  "term_source": "Southern Blot",
  "definition": "1. Southern blotting is a laboratory technique used to detect a specific DNA sequence in a blood or tissue sample. A restriction enzyme is used to cut a sample of DNA into fragments that are separated using gel electrophoresis. The DNA fragments are transferred out of the gel to the surface of a membrane. The membrane is exposed to a DNA probe labeled with a radioactive or chemical tag. If the probe binds to the membrane, then the probe sequence is present in the sample.\n\n2. The separation on an electrophoretic gel of sequences or fragments of genomic or complementary DNA, partially digested by endonucleases, The fragments are then 'blotted' onto a membrane and allowed to hybridize with a specific, labeled probe in order to detect which bands contain the fragment or gene of interest. Southern blot is particularly useful in detecting large gene rearrangements/deletions and large trinucleotide repeat expansions.",
  "subject": "Research",
  "references": [
    "NIH Genetics Home Reference Glossary"
  ],
  "url": "https://mlchart.com/terminology/environment/southern-blot/"
}