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Southern blot

Term · Chemistry · MLC-T-CHM-011065

A molecular biology technique used to detect specific DNA sequences in DNA samples. DNA fragments, separated by gel electrophoresis, are transferred to a membrane and then hybridized with a labeled probe complementary to the target sequence. This method is named after its inventor, Edwin Southern, and is used for genetic mapping, forensic analysis, and disease diagnosis.

Table 1. Record
IdentifierMLC-T-CHM-011065
FieldChemistry
SubjectAnalytical Chemistry
ReferencesPAC, 2018, 90, 1121. 'Terminology of bioanalytical methods (IUPAC Recommendations 2018)' on page 1153 (https://doi.org/10.1515/pac-2016-1120)
See alsoNucleotide sequence
Record as JSON
{
  "id": "MLC-T-CHM-011065",
  "term": "Southern blot",
  "field": "Chemistry",
  "definition": "A molecular biology technique used to detect specific DNA sequences in DNA samples. DNA fragments, separated by gel electrophoresis, are transferred to a membrane and then hybridized with a labeled probe complementary to the target sequence. This method is named after its inventor, Edwin Southern, and is used for genetic mapping, forensic analysis, and disease diagnosis.",
  "subject": "Analytical Chemistry",
  "see_also": [
    "nucleotide sequence"
  ],
  "references": [
    "PAC, 2018, 90, 1121. 'Terminology of bioanalytical methods (IUPAC Recommendations 2018)' on page 1153 (https://doi.org/10.1515/pac-2016-1120)"
  ],
  "url": "https://mlchart.com/terminology/chemistry/southern-blot/"
}

Record 11,787 of 13,676 in Chemistry terminology (MLC-0109). Request the full dataset.