Immunoblotting

{
  "id": "MLC-T-CHM-005657",
  "term": "Immunoblotting",
  "field": "Chemistry",
  "definition": "A laboratory technique used to detect specific proteins in a sample by separating them via gel electrophoresis, transferring them to a membrane, and then probing with antibodies. The primary antibody binds to the target protein, and a secondary antibody, often conjugated with an enzyme or fluorophore, binds to the primary antibody for detection. This method is widely used for protein identification and quantification.",
  "subject": "Analytical Chemistry",
  "references": [
    "PAC, 2018, 90, 1121. 'Terminology of bioanalytical methods (IUPAC Recommendations 2018)' on page 1139 (https://doi.org/10.1515/pac-2016-1120)"
  ],
  "url": "https://mlchart.com/terminology/chemistry/immunoblotting/"
}