Indirect immunofluorescence

{
  "id": "MLC-T-CHM-005780",
  "term": "Indirect immunofluorescence",
  "field": "Chemistry",
  "definition": "A laboratory technique used to detect the presence of specific antibodies or antigens in a sample by employing two antibodies. A primary antibody binds to the target antigen, and then a secondary antibody, conjugated with a fluorescent dye, binds to the primary antibody. This method is widely used in diagnostics for detecting autoimmune diseases or infectious agents.",
  "subject": "Chemistry and Human Health",
  "see_also": [
    "immunoglobulin G"
  ],
  "references": [
    "PAC, 2012, 84, 1113. 'IUPAC glossary of terms used in immunotoxicology (IUPAC Recommendations 2012)' on page 1193 (https://doi.org/10.1351/PAC-REC-11-06-03)"
  ],
  "url": "https://mlchart.com/terminology/chemistry/indirect-immunofluorescence/"
}