Enzyme-linked immunospot assay

{
  "id": "MLC-T-CHM-004004",
  "term": "Enzyme-linked immunospot assay",
  "field": "Chemistry",
  "definition": "A laboratory technique used to detect and quantify cytokine-secreting cells at a single-cell level. Cells are incubated on a membrane coated with an antibody specific for the secreted molecule, which is then detected by a secondary enzyme-linked antibody. This assay provides a quantitative measure of the frequency of specific cytokine-producing cells, often expressed as spot-forming units per million cells.",
  "synonyms": [
    "ELISPOT assay"
  ],
  "subject": "Chemistry and Human Health",
  "see_also": [
    "cytokine",
    "enzyme-linked immunosorbent assay"
  ],
  "references": [
    "PAC, 2012, 84, 1113. 'IUPAC glossary of terms used in immunotoxicology (IUPAC Recommendations 2012)' on page 1161 (https://doi.org/10.1351/PAC-REC-11-06-03)"
  ],
  "url": "https://mlchart.com/terminology/chemistry/enzyme-linked-immunospot-assay/"
}