Noncompetitive binding assay

{
  "id": "MLC-T-CHM-008011",
  "term": "Noncompetitive binding assay",
  "field": "Chemistry",
  "definition": "An analytical method used to quantify a substance by measuring its binding to a specific receptor or antibody, where the labeled and unlabeled forms of the substance do not directly compete for the same binding sites. This assay format often involves sequential additions of reagents, such as in an ELISA (Enzyme-Linked Immunosorbent Assay). The signal generated is directly proportional to the concentration of the analyte.",
  "synonyms": [
    "indirect binding assay"
  ],
  "subject": "Chemistry and Human Health",
  "references": [
    "PAC, 2011, 83, 1129. 'Glossary of terms used in biomolecular screening (IUPAC Recommendations 2011)' on page 1150 (https://doi.org/10.1351/PAC-REC-09-05-03)"
  ],
  "url": "https://mlchart.com/terminology/chemistry/noncompetitive-binding-assay/"
}