Heterogeneous assay

{
  "id": "MLC-T-CHM-005241",
  "term": "Heterogeneous assay",
  "field": "Chemistry",
  "definition": "An analytical method in which the reactants are in different phases, requiring a physical separation step to distinguish between bound and unbound components before measurement. Typically, one component, such as an antibody or antigen, is immobilized on a solid surface like a microplate well, while the other components are in a liquid solution. The enzyme-linked immunosorbent assay (ELISA) is a common example of a heterogeneous assay that requires washing steps to remove unbound reagents.",
  "subject": "Chemistry and Human Health",
  "see_also": [
    "heterogeneous binding assay",
    "homogeneous assay"
  ],
  "references": [
    "PAC, 2011, 83, 1129. 'Glossary of terms used in biomolecular screening (IUPAC Recommendations 2011)' on page 1142 (https://doi.org/10.1351/PAC-REC-09-05-03)"
  ],
  "url": "https://mlchart.com/terminology/chemistry/heterogeneous-assay/"
}