Heterogeneous binding assay

{
  "id": "MLC-T-CHM-005242",
  "term": "Heterogeneous binding assay",
  "field": "Chemistry",
  "definition": "A method for measuring the binding affinity or concentration of a ligand to a receptor, where the bound and unbound species are physically separated. One component is immobilized on a solid phase, allowing for washing steps to remove non-specifically bound molecules. This technique is widely used in drug discovery and clinical diagnostics, often employing radiolabeled or fluorescent ligands.",
  "subject": "Chemistry and Human Health",
  "references": [
    "PAC, 2011, 83, 1129. 'Glossary of terms used in biomolecular screening (IUPAC Recommendations 2011)' on page 1142 (https://doi.org/10.1351/PAC-REC-09-05-03)"
  ],
  "url": "https://mlchart.com/terminology/chemistry/heterogeneous-binding-assay/"
}