Fluorescence polarisation assay

{
  "id": "MLC-T-CHM-004521",
  "term": "Fluorescence polarisation assay",
  "field": "Chemistry",
  "definition": "A homogeneous binding assay that quantifies molecular interactions by measuring changes in the rotational diffusion of a fluorescently labeled molecule upon binding to a larger partner. When a small fluorescent ligand binds to a larger molecule, its rotational speed decreases, leading to an increase in the observed fluorescence polarization. This assay is widely used in high-throughput screening for drug discovery.",
  "subject": "Chemistry and Human Health",
  "see_also": [
    "fluorescence polarisation",
    "homogeneous binding assay"
  ],
  "references": [
    "PAC, 2011, 83, 1129. 'Glossary of terms used in biomolecular screening (IUPAC Recommendations 2011)' on page 1141 (https://doi.org/10.1351/PAC-REC-09-05-03)"
  ],
  "url": "https://mlchart.com/terminology/chemistry/fluorescence-polarisation-assay/"
}