Fluorescence correlation assay

Term · Chemistry · MLC-T-CHM-004517

A technique that measures the concentration and mobility of fluorescently labeled molecules in a solution by analyzing fluctuations in their fluorescence signal. A laser is focused on a tiny observation volume, and as molecules diffuse through it, they emit photons that are detected over time. The resulting autocorrelation function provides information on diffusion coefficients, molecular weight, and binding interactions.

Table 1. Record
Identifier	MLC-T-CHM-004517
Field	Chemistry
Subject	Chemistry and Human Health
References	PAC, 2011, 83, 1129. 'Glossary of terms used in biomolecular screening (IUPAC Recommendations 2011)' on page 1140 (https://doi.org/10.1351/PAC-REC-09-05-03)

Record as JSON

{
  "id": "MLC-T-CHM-004517",
  "term": "Fluorescence correlation assay",
  "field": "Chemistry",
  "definition": "A technique that measures the concentration and mobility of fluorescently labeled molecules in a solution by analyzing fluctuations in their fluorescence signal. A laser is focused on a tiny observation volume, and as molecules diffuse through it, they emit photons that are detected over time. The resulting autocorrelation function provides information on diffusion coefficients, molecular weight, and binding interactions.",
  "subject": "Chemistry and Human Health",
  "references": [
    "PAC, 2011, 83, 1129. 'Glossary of terms used in biomolecular screening (IUPAC Recommendations 2011)' on page 1140 (https://doi.org/10.1351/PAC-REC-09-05-03)"
  ],
  "url": "https://mlchart.com/terminology/chemistry/fluorescence-correlation-assay/"
}

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