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Homogeneous binding assay

Term · Chemistry · MLC-T-CHM-005361

A type of biochemical assay that measures the interaction between two or more molecules in a single solution without requiring physical separation of bound from unbound components. The signal, often fluorescence-based, changes upon binding, allowing direct quantification of the interaction. This method is frequently employed in drug discovery for screening ligand-receptor interactions.

Table 1. Record
IdentifierMLC-T-CHM-005361
FieldChemistry
SubjectChemistry and Human Health
ReferencesPAC, 2011, 83, 1129. 'Glossary of terms used in biomolecular screening (IUPAC Recommendations 2011)' on page 1143 (https://doi.org/10.1351/PAC-REC-09-05-03)
See alsoFluorescence polarisation assay
Record as JSON
{
  "id": "MLC-T-CHM-005361",
  "term": "Homogeneous binding assay",
  "field": "Chemistry",
  "definition": "A type of biochemical assay that measures the interaction between two or more molecules in a single solution without requiring physical separation of bound from unbound components. The signal, often fluorescence-based, changes upon binding, allowing direct quantification of the interaction. This method is frequently employed in drug discovery for screening ligand-receptor interactions.",
  "subject": "Chemistry and Human Health",
  "see_also": [
    "fluorescence polarisation assay"
  ],
  "references": [
    "PAC, 2011, 83, 1129. 'Glossary of terms used in biomolecular screening (IUPAC Recommendations 2011)' on page 1143 (https://doi.org/10.1351/PAC-REC-09-05-03)"
  ],
  "url": "https://mlchart.com/terminology/chemistry/homogeneous-binding-assay/"
}

Record 5,681 of 13,676 in Chemistry terminology (MLC-0109). Request the full dataset.