autologous CD19CAR-CD28-CD137/CD27/CD3zeta-iCasp9-expressing T lymphocytes

{
  "id": "MLC-T-ONC-011539",
  "term": "autologous CD19CAR-CD28-CD137/CD27/CD3zeta-iCasp9-expressing T lymphocytes",
  "field": "Oncology and biomedicine",
  "definition": "Autologous T lymphocytes that have been transduced with a fourth generation-lentiviral vector to express the 4SCAR19 gene composed of a chimeric antigen receptor (CAR) consisting of a single chain variable fragment (scFv) of anti-CD19 coupled to the co-stimulatory molecules CD28, 4-1BB (CD137), and CD27, and to the cytoplasmic portion of the zeta chain of the human T-cell receptor (CD3zeta), and containing the apoptosis-inducible suicide gene human caspase 9 (iCASP9 or iC9), that is linked to a drug binding domain, with potential immunostimulating and antineoplastic activities. The iCASP9 construct consists of the entire coding sequence for the human FK506-drug binding protein (FKBP12) with an F36V mutation (FKBP12-F36V) that is linked to the gene encoding iC9, which is a modified form of the CASP9 gene where the sequences encoding the endogenous caspase activation and recruitment domains have been deleted. Upon transfusion, anti-CD19-CAR-CD28/CD137/CD27/CD3zeta-iCasp9-expressing autologous T lymphocytes target and bind to CD19-expressing neoplastic B cells. This results in a cytotoxic T-lymphocyte (CTL) response against CD19-expressing tumor cells, and causes tumor cell lysis. If the administered T cells cause unacceptable side effects, the chemical homodimerizer AP1903, which binds to the FKBP12-F36V drug-binding domain, can be administered; this induces caspase 9 expression, and results in apoptosis of the administered 4SCAR19 T cells. CD19, cluster of differentiation 19, is a B-cell-specific cell surface antigen overexpressed in B-cell lineage tumors. Incorporation of the costimulatory signaling domains increases human T-cell function, expansion, and survival.",
  "synonyms": [
    "autologous anti-CD19 CAR-T cells 4SCAR19",
    "4S-CAR-T19",
    "4SCAR19 cells",
    "autologous CD19-scFv/CD28/CD137/CD27/CD3z-iCasp9 CAR-T cells",
    "autologous CD19- scFv/CD28/CD137/CD27/CD3z-iCasp9 4SCAR-19 T cells"
  ],
  "references": [
    "National Cancer Institute Thesaurus"
  ],
  "url": "https://mlchart.com/terminology/oncology/autologous-cd19car-cd28-cd137-cd27-cd3zeta-icasp9-expressing-t-lymphocytes/"
}