Epitope retrieval

{
  "id": "MLC-T-CHM-004044",
  "term": "Epitope retrieval",
  "field": "Chemistry",
  "definition": "A laboratory procedure employed to unmask or expose antigenic sites in tissue sections, particularly after fixation with agents like formalin. This treatment reverses the cross-linking and conformational changes induced by fixatives, allowing antibodies to bind to their target epitopes. Heat-induced epitope retrieval (HIER) and enzyme-induced epitope retrieval (EIER) are two common approaches.",
  "synonyms": [
    "antigen retrieval"
  ],
  "subject": "Chemistry and Human Health",
  "references": [
    "PAC, 2012, 84, 1113. 'IUPAC glossary of terms used in immunotoxicology (IUPAC Recommendations 2012)' on page 1162 (https://doi.org/10.1351/PAC-REC-11-06-03)"
  ],
  "url": "https://mlchart.com/terminology/chemistry/epitope-retrieval/"
}