Stable isotope ratio analysis of amino acids in cell culture

{
  "id": "MLC-T-CHM-011268",
  "term": "Stable isotope ratio analysis of amino acids in cell culture",
  "field": "Chemistry",
  "definition": "A metabolic labelling approach in which cultured cells grow in medium containing amino acids enriched in stable isotopes, such as 13C- or 15N-labelled lysine and arginine, so that newly made proteins carry a known mass shift. Labelled and unlabelled cell populations are mixed and analysed by mass spectrometry, and peptide peak intensity ratios give relative protein abundance. Heavy and light forms of a labelled peptide typically differ by several daltons.",
  "subject": "Analytical Chemistry",
  "references": [
    "PAC, 2013, 85, 1515. 'Definitions of terms relating to mass spectrometry (IUPAC Recommendations 2013)' on page 1592 (https://doi.org/10.1351/PAC-REC-06-04-06)"
  ],
  "url": "https://mlchart.com/terminology/chemistry/stable-isotope-ratio-analysis-of-amino-acids-in-cell-culture/"
}