Stable isotope standards and capture by anti-peptide antibodies

{
  "id": "MLC-T-CHM-011269",
  "term": "Stable isotope standards and capture by anti-peptide antibodies",
  "field": "Chemistry",
  "definition": "A quantitative proteomics method that uses synthetic peptides labeled with stable isotopes as internal standards, which are then spiked into a biological sample. These labeled peptides, along with their endogenous counterparts, are subsequently captured by specific anti-peptide antibodies. The ratio of labeled to unlabeled peptides is then determined by mass spectrometry to quantify protein abundance.",
  "subject": "Analytical Chemistry",
  "see_also": [
    "surrogate internal standard"
  ],
  "references": [
    "PAC, 2013, 85, 1515. 'Definitions of terms relating to mass spectrometry (IUPAC Recommendations 2013)' on page 1592 (https://doi.org/10.1351/PAC-REC-06-04-06)"
  ],
  "url": "https://mlchart.com/terminology/chemistry/stable-isotope-standards-and-capture-by-anti-peptide-antibodies/"
}