Quantitation by concatenated tryptic peptides

{
  "id": "MLC-T-CHM-013458",
  "term": "Quantitation by concatenated tryptic peptides",
  "field": "Chemistry",
  "definition": "A method for absolute protein quantitation in mass spectrometry that involves creating a synthetic protein standard by concatenating multiple tryptic peptides. This standard, often isotopically labeled, is added to a sample before digestion and analysis. The ratio of the labeled standard peptides to their endogenous counterparts provides a direct measure of the protein's concentration.",
  "subject": "Analytical Chemistry",
  "see_also": [
    "surrogate internal standard"
  ],
  "references": [
    "PAC, 2013, 85, 1515. 'Definitions of terms relating to mass spectrometry (IUPAC Recommendations 2013)' on page 1581 (https://doi.org/10.1351/PAC-REC-06-04-06)"
  ],
  "url": "https://mlchart.com/terminology/chemistry/quantitation-by-concatenated-tryptic-peptides/"
}