Isotype control

{
  "id": "MLC-T-CHM-006267",
  "term": "Isotype control",
  "field": "Chemistry",
  "definition": "An antibody of the same immunoglobulin class, subclass, and light chain type as a primary antibody, but lacking specificity for the target antigen. It is used in immunoassays, particularly flow cytometry, to differentiate non-specific background staining from true antigen-specific signals. For a valid comparison, the isotype control antibody must be from the same species and used at the same concentration as the primary experimental antibody.",
  "subject": "Chemistry and Human Health",
  "see_also": [
    "flow cytometry",
    "isotype"
  ],
  "references": [
    "PAC, 2012, 84, 1113. 'IUPAC glossary of terms used in immunotoxicology (IUPAC Recommendations 2012)' on page 1197 (https://doi.org/10.1351/PAC-REC-11-06-03)"
  ],
  "url": "https://mlchart.com/terminology/chemistry/isotype-control/"
}