JAM test

{
  "id": "MLC-T-CHM-006279",
  "term": "JAM test",
  "field": "Chemistry",
  "definition": "A cytotoxicity assay in which target cells are labeled with tritiated thymidine incorporated into their DNA and then mixed with effector cells such as cytotoxic T lymphocytes or natural killer cells. Killing by apoptosis fragments the target DNA, and the fragments are lost when cells are harvested onto filters, so a drop in retained radioactivity measures killing. Matzinger introduced it in 1991 as an alternative to chromium release.",
  "subject": "Chemistry and Human Health",
  "see_also": [
    "cell-mediated cytotoxicity"
  ],
  "references": [
    "PAC, 2012, 84, 1113. 'IUPAC glossary of terms used in immunotoxicology (IUPAC Recommendations 2012)' on page 1197 (https://doi.org/10.1351/PAC-REC-11-06-03)"
  ],
  "url": "https://mlchart.com/terminology/chemistry/jam-test/"
}