Sanger sequencing

{
  "id": "MLC-T-CHM-010512",
  "term": "Sanger sequencing",
  "field": "Chemistry",
  "definition": "A method for determining the nucleotide sequence of DNA, based on the selective incorporation of chain-terminating dideoxynucleotides during DNA replication. This technique generates DNA fragments of varying lengths, which are then separated by size to reveal the sequence. It was a foundational method for genome sequencing.",
  "subject": "Analytical Chemistry",
  "see_also": [
    "nucleotide sequence"
  ],
  "references": [
    "PAC, 2018, 90, 1121. 'Terminology of bioanalytical methods (IUPAC Recommendations 2018)' on page 1152 (https://doi.org/10.1515/pac-2016-1120)"
  ],
  "url": "https://mlchart.com/terminology/chemistry/sanger-sequencing/"
}