Self-absorption effect

{
  "id": "MLC-T-CHM-010700",
  "term": "Self-absorption effect",
  "field": "Chemistry",
  "definition": "The reduction in the observed intensity of emitted radiation due to reabsorption by unexcited species of the same type within the emitting medium. This effect is particularly relevant in luminescence spectroscopy, where emitted photons can be reabsorbed by other fluorophores before exiting the sample. It can lead to non-linear calibration curves at higher analyte concentrations.",
  "subject": "Analytical Chemistry",
  "see_also": [
    "luminescence"
  ],
  "references": [
    "PAC, 1984, 56, 231. 'Nomenclature, symbols, units and their usage in spectrochemical analysis-Part VI: molecular luminescence spectroscopy' on page 244 (https://doi.org/10.1351/pac198456020231)"
  ],
  "url": "https://mlchart.com/terminology/chemistry/self-absorption-effect/"
}