MLchartDataset catalogue

Affinity chromatography

Term · Chemistry · MLC-T-CHM-000317

A chromatographic separation technique that exploits the specific binding affinity between an analyte and a ligand immobilized on a stationary phase. Target molecules selectively bind to the ligand, while other components pass through the column. Elution of the bound analyte is achieved by changing conditions such as pH, ionic strength, or by adding a competing ligand.

Table 1. Record
IdentifierMLC-T-CHM-000317
FieldChemistry
SubjectAnalytical Chemistry
ReferencesPAC, 1993, 65, 819. 'Nomenclature for chromatography (IUPAC Recommendations 1993)' on page 826 (https://doi.org/10.1351/pac199365040819)
See alsoChromatography
Record as JSON 
{
  "id": "MLC-T-CHM-000317",
  "term": "Affinity chromatography",
  "field": "Chemistry",
  "definition": "A chromatographic separation technique that exploits the specific binding affinity between an analyte and a ligand immobilized on a stationary phase. Target molecules selectively bind to the ligand, while other components pass through the column. Elution of the bound analyte is achieved by changing conditions such as pH, ionic strength, or by adding a competing ligand.",
  "subject": "Analytical Chemistry",
  "see_also": [
    "chromatography"
  ],
  "references": [
    "PAC, 1993, 65, 819. 'Nomenclature for chromatography (IUPAC Recommendations 1993)' on page 826 (https://doi.org/10.1351/pac199365040819)"
  ],
  "url": "https://mlchart.com/terminology/chemistry/affinity-chromatography/"
}

Record 337 of 13,676 in Chemistry terminology (MLC-0109). Request the full dataset.