Isoelectric focusing

{
  "id": "MLC-T-CHM-006180",
  "term": "Isoelectric focusing",
  "field": "Chemistry",
  "definition": "A high-resolution electrophoretic technique that separates proteins or peptides based on their isoelectric points (pI). Molecules migrate through a pH gradient in an electric field until they reach the position where their net charge is zero, at which point they stop moving. This method is often the first dimension in two-dimensional gel electrophoresis.",
  "subject": "Analytical Chemistry",
  "see_also": [
    "gel electrophoresis"
  ],
  "references": [
    "PAC, 2018, 90, 1121. 'Terminology of bioanalytical methods (IUPAC Recommendations 2018)' on page 1156 (https://doi.org/10.1515/pac-2016-1120)"
  ],
  "url": "https://mlchart.com/terminology/chemistry/isoelectric-focusing/"
}