Two-dimensional gel electrophoresis

{
  "id": "MLC-T-CHM-012376",
  "term": "Two-dimensional gel electrophoresis",
  "field": "Chemistry",
  "definition": "A high-resolution analytical technique used to separate proteins based on two independent properties: isoelectric point and molecular weight. Proteins are first separated by isoelectric focusing in a pH gradient, then by size using sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE). This method can resolve thousands of proteins from a complex biological sample on a single gel.",
  "subject": "Analytical Chemistry",
  "see_also": [
    "gel electrophoresis",
    "isoelectric focusing"
  ],
  "references": [
    "PAC, 2018, 90, 1121. 'Terminology of bioanalytical methods (IUPAC Recommendations 2018)' on page 1165 (https://doi.org/10.1515/pac-2016-1120)"
  ],
  "url": "https://mlchart.com/terminology/chemistry/two-dimensional-gel-electrophoresis/"
}