Off-gel isoelectric focusing

{
  "id": "MLC-T-CHM-008200",
  "term": "Off-gel isoelectric focusing",
  "field": "Chemistry",
  "definition": "A method for separating proteins or peptides based on their isoelectric points, performed in solution rather than within a gel matrix. The sample is placed in individual wells containing immobilized pH gradient strips, and an electric field is applied to drive the molecules to their pI. This technique is often used as a prefractionation step before mass spectrometry.",
  "subject": "Analytical Chemistry",
  "see_also": [
    "isoelectric focusing"
  ],
  "references": [
    "PAC, 2018, 90, 1121. 'Terminology of bioanalytical methods (IUPAC Recommendations 2018)' on page 1157 (https://doi.org/10.1515/pac-2016-1120)"
  ],
  "url": "https://mlchart.com/terminology/chemistry/off-gel-isoelectric-focusing/"
}