Post-column derivatization

{
  "id": "MLC-T-CHM-009302",
  "term": "Post-column derivatization",
  "field": "Chemistry",
  "definition": "A chemical reaction performed on analytes after they have been separated by a chromatographic column but before they reach the detector. This process converts the analytes into derivatives that possess properties more suitable for detection, such as enhanced fluorescence or UV absorbance. A common application is the ninhydrin reaction for amino acid detection in high-performance liquid chromatography.",
  "subject": "Analytical Chemistry",
  "see_also": [
    "reaction chromatography"
  ],
  "references": [
    "PAC, 1993, 65, 819. 'Nomenclature for chromatography (IUPAC Recommendations 1993)' on page 827 (https://doi.org/10.1351/pac199365040819)"
  ],
  "url": "https://mlchart.com/terminology/chemistry/post-column-derivatization/"
}