Charge site derivatization

{
  "id": "MLC-T-CHM-001907",
  "term": "Charge site derivatization",
  "field": "Chemistry",
  "definition": "A chemical modification of an analyte to introduce a permanent, localized charge at a specific location within the molecule. This derivatization directs fragmentation in tandem mass spectrometry by creating a fixed charge site, which is not the mobile proton that typically initiates fragmentation pathways. For example, the quaternization of a tertiary amine with an alkyl iodide introduces a fixed positive charge on the nitrogen atom, altering subsequent fragmentation patterns.",
  "subject": "Analytical Chemistry",
  "references": [
    "PAC, 2013, 85, 1515. 'Definitions of terms relating to mass spectrometry (IUPAC Recommendations 2013)' on page 1529 (https://doi.org/10.1351/PAC-REC-06-04-06)"
  ],
  "url": "https://mlchart.com/terminology/chemistry/charge-site-derivatization/"
}