Proteolytic digestion

{
  "id": "MLC-T-CHM-009599",
  "term": "Proteolytic digestion",
  "field": "Chemistry",
  "definition": "The controlled enzymatic cleavage of proteins into smaller peptide fragments using specific proteases. This process is a fundamental step in bottom-up proteomics, preparing proteins for analysis by mass spectrometry. Trypsin is a commonly used enzyme due to its high specificity for cleaving after lysine and arginine residues.",
  "subject": "Analytical Chemistry",
  "see_also": [
    "proteolysis"
  ],
  "references": [
    "PAC, 2018, 90, 1121. 'Terminology of bioanalytical methods (IUPAC Recommendations 2018)' on page 1163 (https://doi.org/10.1515/pac-2016-1120)"
  ],
  "url": "https://mlchart.com/terminology/chemistry/proteolytic-digestion/"
}