Quantitative polymerase chain reaction

{
  "id": "MLC-T-CHM-009750",
  "term": "Quantitative polymerase chain reaction",
  "field": "Chemistry",
  "definition": "A molecular biology technique that measures the amplification of a targeted DNA molecule in real-time during the polymerase chain reaction (PCR). This method allows for the quantification of specific DNA or RNA sequences in a sample. The results are typically expressed as a cycle threshold (Ct) value, inversely proportional to the initial amount of target nucleic acid.",
  "synonyms": [
    "quantitative PCR"
  ],
  "subject": "Analytical Chemistry",
  "references": [
    "PAC, 2018, 90, 1121. 'Terminology of bioanalytical methods (IUPAC Recommendations 2018)' on page 1151 (https://doi.org/10.1515/pac-2016-1120)"
  ],
  "url": "https://mlchart.com/terminology/chemistry/quantitative-polymerase-chain-reaction/"
}