Real-time polymerase chain reaction

{
  "id": "MLC-T-CHM-009996",
  "term": "Real-time polymerase chain reaction",
  "field": "Chemistry",
  "definition": "A molecular biology technique that monitors the amplification of deoxyribonucleic acid (DNA) during the polymerase chain reaction (PCR) in real time. This method quantifies the initial amount of DNA by measuring fluorescence emitted during each cycle. The results are expressed as a cycle threshold (Ct) value.",
  "synonyms": [
    "real-time PCR"
  ],
  "subject": "Analytical Chemistry",
  "see_also": [
    "quantitative polymerase chain reaction"
  ],
  "references": [
    "PAC, 2018, 90, 1121. 'Terminology of bioanalytical methods (IUPAC Recommendations 2018)' on page 1151 (https://doi.org/10.1515/pac-2016-1120)"
  ],
  "url": "https://mlchart.com/terminology/chemistry/real-time-polymerase-chain-reaction/"
}